nikon_eclipse_ti
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nikon_eclipse_ti [2020/03/01 17:36] bioimaging [Objectives] |
nikon_eclipse_ti [2021/03/27 00:57] bioimaging |
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| <b>Model</b>: Eclipse Ti<br> | <b>Model</b>: Eclipse Ti<br> | ||
| <b>Nickname</b>: "006"<br> | <b>Nickname</b>: "006"<br> | ||
| - | <b>Software</b>: Metamorph / NIS-Elements AR 5.11.01<br> | + | <b>Software</b>: NIS-Elements AR 5.11.01<br> |
| <b>Year</b>: 2010 <br> | <b>Year</b>: 2010 <br> | ||
| <b>SN</b>: 602805 <br> | <b>SN</b>: 602805 <br> | ||
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|   <br> |   <br> | ||
|   <br> |   <br> | ||
| - | → <a href="https://imm.medicina.ulisboa.pt/intranet/booking/163"><img src="/facility/bioimaging/lib/exe/fetch.php?media=date.png"> Nikon Eclipse Ti Booking</a> | + | → <a href="https://my.agendo.science/calendar/?id=MzhjYWxlbmRhcnM="><img src="/facility/bioimaging/lib/exe/fetch.php?media=date.png"> Nikon Eclipse Ti Booking</a> |
| <br> | <br> | ||
| → <a href="/facility/bioimaging/doku.php?id=nikon_eclipse_ti_usage"><img src="/facility/bioimaging/lib/exe/fetch.php?media=chart_line.png"> Nikon Eclipse Ti Usage Statistics</a> | → <a href="/facility/bioimaging/doku.php?id=nikon_eclipse_ti_usage"><img src="/facility/bioimaging/lib/exe/fetch.php?media=chart_line.png"> Nikon Eclipse Ti Usage Statistics</a> | ||
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| * **Microscope**: [[https://www.microscope.healthcare.nikon.com/en_EU/products/inverted-microscopes/eclipse-ti-series|Nikon Eclipse Ti]] | * **Microscope**: [[https://www.microscope.healthcare.nikon.com/en_EU/products/inverted-microscopes/eclipse-ti-series|Nikon Eclipse Ti]] | ||
| - | * **Camera**: [[https://www.qimaging.com/retiga-r6|Teledyne QImaging Retiga R6 mono CCD]] | + | * **Camera**: [[https://andor.oxinst.com/products/scmos-camera-series/neo-5-5-scmos|Andor Neo 5.5 sCMOS]] |
| {{:warning.png?25|}} Data files older than **3 months** will be automatically deleted on this system, please copy your data to the iMM server using the desktop link. | {{:warning.png?25|}} Data files older than **3 months** will be automatically deleted on this system, please copy your data to the iMM server using the desktop link. | ||
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| ^ Position ^ Filterset ^ Reference ^ Excitation ^ Dichroic ^ Emission ^ | ^ Position ^ Filterset ^ Reference ^ Excitation ^ Dichroic ^ Emission ^ | ||
| - | | 1 | none | none | none | none | none | | + | | 1 | Empty ||||| |
| | 2 | DAPI | MBE41300 | 340-380 nm | 400 nm | 435-485 nm | | | 2 | DAPI | MBE41300 | 340-380 nm | 400 nm | 435-485 nm | | ||
| | 3 | FITC | MBE44720 | 465-495 nm | 505 nm | 515-555 nm | | | 3 | FITC | MBE44720 | 465-495 nm | 505 nm | 515-555 nm | | ||
| | 4 | TRITC | MBE45600 | 528-553 nm | 565 nm | 590-650 nm | | | 4 | TRITC | MBE45600 | 528-553 nm | 565 nm | 590-650 nm | | ||
| | 5 | Cy5 | 2SK4CY50 | 608-648 nm | 660 nm | 672-712 nm | | | 5 | Cy5 | 2SK4CY50 | 608-648 nm | 660 nm | 672-712 nm | | ||
| - | | 6 | none | none | none | none | none | | + | | 6 | Empty ||||| |
| ==== Objectives ==== | ==== Objectives ==== | ||
| ^ Magnification ^ Model ^ Immersion ^ NA ^ WD (mm) ^ Reference ^ | ^ Magnification ^ Model ^ Immersion ^ NA ^ WD (mm) ^ Reference ^ | ||
| - | | 10x | CFI Plan Fluor DLL Ph1 | Air | 0.30 | 16.00 | [[https://www.microscope.healthcare.nikon.com/en_EU/selectors/objective-comparison/-1835|MRH10101]] | | + | | 10x | CFI Plan Fluor DLL Ph1 | Air | 0.30 | 16.00 | [[https://www.microscope.healthcare.nikon.com/en_EU/selectors/objective-comparison/-1835|MRH10101]] | |
| + | | 20x | CFI S Plan Fluor ELWD ADM Ph1 | Air | 0.45 | 6.90 - 8.20 | [[https://www.microscope.healthcare.nikon.com/en_EU/selectors/objective-comparison/-1813|MRH48230]] | | ||
| | 40x | CFI S Plan Fluor ELWD ADM Ph2 | Air | 0.60 | 2.80 - 3.60 | [[https://www.microscope.healthcare.nikon.com/en_EU/selectors/objective-comparison/-1814|MRH48430]] | | | 40x | CFI S Plan Fluor ELWD ADM Ph2 | Air | 0.60 | 2.80 - 3.60 | [[https://www.microscope.healthcare.nikon.com/en_EU/selectors/objective-comparison/-1814|MRH48430]] | | ||
| - | | 40x | CFI Plan Apo DM Ph3 | Water | 1.00 | 0.16 | | | + | | 40x | Plan Apo DM Ph3 | Oil | 1.00 | 0.16 | | |
| **Upon request:** | **Upon request:** | ||
| - | ^ Magnification ^ Model ^ Immersion ^ NA ^ WD (mm) ^ Reference ^ | + | ^ Magnification ^ Model ^ Immersion ^ NA ^ WD (mm) ^ pixel size* (μm) ^ 10 μm = ^ Reference ^ |
| - | | 40x | CFI S Plan Fluor ELWD ADL Ph2 | Air | 0.60 | 2.70 - 3.70 | | | + | | 40x | Plan Fluor ELWD ADL Ph2 | Air | 0.60 | 2.70 - 3.70 | 0.113 | 88 pixels | | |
| - | | 100x | CFI Plan | Water | 1.10 | 2.50 | [[https://www.microscope.healthcare.nikon.com/en_EU/selectors/objective-comparison/-1956|MRL07920]] | | + | |
| + | *** pixel sizes for binning = 1** | ||
| ==== Camera ==== | ==== Camera ==== | ||
| ^ Model ^ Type ^ Frame Size ^ Pixel Size (µm) ^ | ^ Model ^ Type ^ Frame Size ^ Pixel Size (µm) ^ | ||
| - | | [[https://www.qimaging.com/retiga-r6|Retiga R6 mono CCD]] | CCD | 2688 x 2200 | 4.54 x 4.54 | | + | | [[https://andor.oxinst.com/products/scmos-camera-series/neo-5-5-scmos|Andor Neo 5.5 sCMOS]] | CCD | 2560 x 2160 | 6.5 x 6.5 | |
| ---- | ---- | ||
| - | [[resources|Back to the Resources page]] | + | [[resources|Back to the Equipment page]] |
nikon_eclipse_ti.txt · Last modified: 2024/02/12 22:47 by bioimaging
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