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nikon_eclipse_ti [2020/01/01 22:16] bioimaging |
nikon_eclipse_ti [2021/04/05 23:40] bioimaging |
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<b>Model</b>: Eclipse Ti<br> | <b>Model</b>: Eclipse Ti<br> | ||
<b>Nickname</b>: "006"<br> | <b>Nickname</b>: "006"<br> | ||
- | <b>Software</b>: Metamorph / NIS-Elements<br> | + | <b>Software</b>: NIS-Elements AR 5.11.01<br> |
- | <b>Year</b>: 2007 <br> | + | <b>Year</b>: 2010 <br> |
<b>SN</b>: 602805 <br> | <b>SN</b>: 602805 <br> | ||
  <br> |   <br> | ||
  <br> |   <br> | ||
  <br> |   <br> | ||
- |   <br> | + | → <a href="https://my.agendo.science/calendar/?id=MzhjYWxlbmRhcnM="><img src="/facility/bioimaging/lib/exe/fetch.php?media=date.png"> Nikon Eclipse Ti Booking</a> |
- | → <a href="https://imm.medicina.ulisboa.pt/intranet/booking/163"><img src="/facility/bioimaging/lib/exe/fetch.php?media=date.png"> Nikon Eclipse Ti Booking</a> | + | <br> |
+ | → <a href="/facility/bioimaging/doku.php?id=nikon_eclipse_ti_quality"><img src="/facility/bioimaging/lib/exe/fetch.php?media=list-icon.png" width=18> Nikon Eclipse Ti Quality Control</a> | ||
<br> | <br> | ||
→ <a href="/facility/bioimaging/doku.php?id=nikon_eclipse_ti_usage"><img src="/facility/bioimaging/lib/exe/fetch.php?media=chart_line.png"> Nikon Eclipse Ti Usage Statistics</a> | → <a href="/facility/bioimaging/doku.php?id=nikon_eclipse_ti_usage"><img src="/facility/bioimaging/lib/exe/fetch.php?media=chart_line.png"> Nikon Eclipse Ti Usage Statistics</a> | ||
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</table> | </table> | ||
</html> | </html> | ||
+ | |||
+ | ===== Microscope overview ===== | ||
+ | {{ :widefield_lightpath.png?0x300}}The Nikon Eclipse Ti is a fully motorized inverted //widefield fluorescence microscope// ideal for live-cell imaging applications. It is equipped with a small stage incubator for temperature control and CO2 supply and with Perfect Focus System for hardware focus control during long time-lapse acquisitions. Using either Metamorph or Nikon Elements software you can control the motorized filters, shutters and stage to simultaneously set up multi-color time-lapse imaging experiments in multiple stage positions. It is equipped with a Retiga R6 monochrome CCD camera that allows for a large field of view, ideal for time-lapse and tile-and-stitch microscopy. You can perform Z-stack acquisition and use [[https://huygens.i3s.up.pt/login.php|PPBI Huygens RM]] [[http://micro.magnet.fsu.edu/primer/digitalimaging/deconvolution/deconvolutionhome.html|deconvolution]] server to significantly improve image resolution even in thick samples. If don't want to use deconvolution, check out one of the confocal systems such as the point scanners [[zeiss_lsm_710|Zeiss LSM 710]] and [[zeiss_lsm_880|Zeiss LSM 880]] or spinning disks [[3i_marianas_sdc|3i Marianas SDC]] and [[zeiss_cell_observer_sd|Zeiss Cell Observer SD]]. If your personal computer cannot handle all the data you collected, check out the [[big_guy|Big Guy]] or [[colossus|Colossus]]. | ||
+ | |||
+ | * **Microscope**: [[https://www.microscope.healthcare.nikon.com/en_EU/products/inverted-microscopes/eclipse-ti-series|Nikon Eclipse Ti]] | ||
+ | * **Camera**: [[https://andor.oxinst.com/products/scmos-camera-series/neo-5-5-scmos|Andor Neo 5.5 sCMOS]] | ||
+ | |||
+ | {{:warning.png?25|}} Data files older than **3 months** will be automatically deleted on this system, please copy your data to the iMM server using the desktop link. | ||
+ | |||
+ | ===== System components ===== | ||
+ | |||
+ | ==== Filtersets ==== | ||
+ | |||
+ | ^ Position ^ Filterset ^ Reference ^ Excitation ^ Dichroic ^ Emission ^ | ||
+ | | 1 | Empty ||||| | ||
+ | | 2 | DAPI | MBE41300 | 340-380 nm | 400 nm | 435-485 nm | | ||
+ | | 3 | FITC | MBE44720 | 465-495 nm | 505 nm | 515-555 nm | | ||
+ | | 4 | TRITC | MBE45600 | 528-553 nm | 565 nm | 590-650 nm | | ||
+ | | 5 | Cy5 | 2SK4CY50 | 608-648 nm | 660 nm | 672-712 nm | | ||
+ | | 6 | Empty ||||| | ||
+ | |||
+ | ==== Objectives ==== | ||
+ | ^ Magnification ^ Model ^ Immersion ^ NA ^ WD (mm) ^ Reference ^ | ||
+ | | 10x | CFI Plan Fluor DLL Ph1 | Air | 0.30 | 16.00 | [[https://www.microscope.healthcare.nikon.com/en_EU/selectors/objective-comparison/-1835|MRH10101]] | | ||
+ | | 20x | CFI S Plan Fluor ELWD ADM Ph1 | Air | 0.45 | 6.90 - 8.20 | [[https://www.microscope.healthcare.nikon.com/en_EU/selectors/objective-comparison/-1813|MRH48230]] | | ||
+ | | 40x | CFI S Plan Fluor ELWD ADM Ph2 | Air | 0.60 | 2.80 - 3.60 | [[https://www.microscope.healthcare.nikon.com/en_EU/selectors/objective-comparison/-1814|MRH48430]] | | ||
+ | | 40x | Plan Apo DM Ph3 | Oil | 1.00 | 0.16 | | | ||
+ | |||
+ | **Upon request:** | ||
+ | ^ Magnification ^ Model ^ Immersion ^ NA ^ WD (mm) ^ pixel size* (μm) ^ 10 μm = ^ Reference ^ | ||
+ | | 40x | Plan Fluor ELWD ADL Ph2 | Air | 0.60 | 2.70 - 3.70 | 0.113 | 88 pixels | | | ||
+ | |||
+ | |||
+ | *** pixel sizes for binning = 1** | ||
+ | ==== Camera ==== | ||
+ | ^ Model ^ Type ^ Frame Size ^ Pixel Size (µm) ^ | ||
+ | | [[https://andor.oxinst.com/products/scmos-camera-series/neo-5-5-scmos|Andor Neo 5.5 sCMOS]] | CCD | 2560 x 2160 | 6.5 x 6.5 | | ||
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- | [[resources|Back to the Resources page]] | + | [[resources|Back to the Equipment page]] |