nikon_eclipse_ti
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nikon_eclipse_ti [2019/12/31 02:43] bioimaging created |
nikon_eclipse_ti [2020/03/01 18:05] bioimaging [Filtersets] |
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| <a href="/facility/bioimaging/lib/exe/fetch.php?media=nikon_eclipse_ti_wiki_new.png"><img src="/facility/bioimaging/lib/exe/fetch.php?media=nikon_eclipse_ti_wiki_new.png" width=300></a> | <a href="/facility/bioimaging/lib/exe/fetch.php?media=nikon_eclipse_ti_wiki_new.png"><img src="/facility/bioimaging/lib/exe/fetch.php?media=nikon_eclipse_ti_wiki_new.png" width=300></a> | ||
| <td style="border:0px solid white;"> <p style="line-height:1.8"> | <td style="border:0px solid white;"> <p style="line-height:1.8"> | ||
| - | <b>Location</b>: Room P3-A-5 <br> | + | <b>Location</b>: <a href="/facility/bioimaging/lib/exe/fetch.php?media=nikon_eclipse_ti_map_new.png">Room P3-A-5</a> <br> |
| <b>Manufacturer</b>: <a href="https://www.microscope.healthcare.nikon.com/en_EU/" target="_blank">Nikon Instruments</a><br> | <b>Manufacturer</b>: <a href="https://www.microscope.healthcare.nikon.com/en_EU/" target="_blank">Nikon Instruments</a><br> | ||
| <b>Model</b>: Eclipse Ti<br> | <b>Model</b>: Eclipse Ti<br> | ||
| <b>Nickname</b>: "006"<br> | <b>Nickname</b>: "006"<br> | ||
| - | <b>Software</b>: Metamorph / NIS-Elements<br> | + | <b>Software</b>: Metamorph / NIS-Elements AR 5.11.01<br> |
| - | <b>Year</b>: 2007 <br> | + | <b>Year</b>: 2010 <br> |
| <b>SN</b>: 602805 <br> | <b>SN</b>: 602805 <br> | ||
|   <br> |   <br> | ||
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| </table> | </table> | ||
| </html> | </html> | ||
| + | |||
| + | ===== Microscope overview ===== | ||
| + | {{ :widefield_lightpath.png?0x300}}The Nikon Eclipse Ti is a fully motorized inverted //widefield fluorescence microscope// ideal for live-cell imaging applications. It is equipped with a small stage incubator for temperature control and CO2 supply and with Perfect Focus System for hardware focus control during long time-lapse acquisitions. Using either Metamorph or Nikon Elements software you can control the motorized filters, shutters and stage to simultaneously set up multi-color time-lapse imaging experiments in multiple stage positions. It is equipped with a Retiga R6 monochrome CCD camera that allows for a large field of view, ideal for time-lapse and tile-and-stitch microscopy. You can perform Z-stack acquisition and use [[https://huygens.i3s.up.pt/login.php|PPBI Huygens RM]] [[http://micro.magnet.fsu.edu/primer/digitalimaging/deconvolution/deconvolutionhome.html|deconvolution]] server to significantly improve image resolution even in thick samples. If don't want to use deconvolution, check out one of the confocal systems such as the point scanners [[zeiss_lsm_710|Zeiss LSM 710]] and [[zeiss_lsm_880|Zeiss LSM 880]] or spinning disks [[3i_marianas_sdc|3i Marianas SDC]] and [[zeiss_cell_observer_sd|Zeiss Cell Observer SD]]. If your personal computer cannot handle all the data you collected, check out the [[big_guy|Big Guy]] or [[colossus|Colossus]]. | ||
| + | |||
| + | * **Microscope**: [[https://www.microscope.healthcare.nikon.com/en_EU/products/inverted-microscopes/eclipse-ti-series|Nikon Eclipse Ti]] | ||
| + | * **Camera**: [[https://www.qimaging.com/retiga-r6|Teledyne QImaging Retiga R6 mono CCD]] | ||
| + | |||
| + | {{:warning.png?25|}} Data files older than **3 months** will be automatically deleted on this system, please copy your data to the iMM server using the desktop link. | ||
| + | |||
| + | ===== System components ===== | ||
| + | |||
| + | ==== Filtersets ==== | ||
| + | |||
| + | ^ Position ^ Filterset ^ Reference ^ Excitation ^ Dichroic ^ Emission ^ | ||
| + | | 1 | Empty ||||| | ||
| + | | 2 | DAPI | MBE41300 | 340-380 nm | 400 nm | 435-485 nm | | ||
| + | | 3 | FITC | MBE44720 | 465-495 nm | 505 nm | 515-555 nm | | ||
| + | | 4 | TRITC | MBE45600 | 528-553 nm | 565 nm | 590-650 nm | | ||
| + | | 5 | Cy5 | 2SK4CY50 | 608-648 nm | 660 nm | 672-712 nm | | ||
| + | | 6 |||||| | ||
| + | |||
| + | ==== Objectives ==== | ||
| + | ^ Magnification ^ Model ^ Immersion ^ NA ^ WD (mm) ^ Reference ^ | ||
| + | | 10x | CFI Plan Fluor DLL Ph1 | Air | 0.30 | 16.00 | [[https://www.microscope.healthcare.nikon.com/en_EU/selectors/objective-comparison/-1835|MRH10101]] | | ||
| + | | 40x | CFI S Plan Fluor ELWD ADM Ph2 | Air | 0.60 | 2.80 - 3.60 | [[https://www.microscope.healthcare.nikon.com/en_EU/selectors/objective-comparison/-1814|MRH48430]] | | ||
| + | | 40x | CFI Plan Apo DM Ph3 | Water | 1.00 | 0.16 | | | ||
| + | |||
| + | **Upon request:** | ||
| + | ^ Magnification ^ Model ^ Immersion ^ NA ^ WD (mm) ^ Reference ^ | ||
| + | | 40x | CFI S Plan Fluor ELWD ADL Ph2 | Air | 0.60 | 2.70 - 3.70 | | | ||
| + | | 100x | CFI Plan | Water | 1.10 | 2.50 | [[https://www.microscope.healthcare.nikon.com/en_EU/selectors/objective-comparison/-1956|MRL07920]] | | ||
| + | |||
| + | ==== Camera ==== | ||
| + | ^ Model ^ Type ^ Frame Size ^ Pixel Size (µm) ^ | ||
| + | | [[https://www.qimaging.com/retiga-r6|Retiga R6 mono CCD]] | CCD | 2688 x 2200 | 4.54 x 4.54 | | ||
| ---- | ---- | ||
| [[resources|Back to the Resources page]] | [[resources|Back to the Resources page]] | ||
nikon_eclipse_ti.txt · Last modified: 2024/02/12 22:47 by bioimaging
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