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Both sides previous revision Previous revision Next revision | Previous revision Next revision Both sides next revision | ||
leica_sp8_mp [2020/03/01 16:00] bioimaging [Objectives] |
leica_sp8_mp [2021/01/17 04:44] bioimaging [Microscope Turn Off procedures] |
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<b>Nickname</b>: "Multiphoton"<br> | <b>Nickname</b>: "Multiphoton"<br> | ||
<b>Software</b>: LAS X<br> | <b>Software</b>: LAS X<br> | ||
- | <b>Software version</b>: 3.5.5.19976<br> | + | <b>Software version</b>: 3.5.7<br> |
<b>Year</b>: 2017 <br> | <b>Year</b>: 2017 <br> | ||
<b>SN</b>: 8100001758 <br> | <b>SN</b>: 8100001758 <br> | ||
  <br> |   <br> | ||
  <br> |   <br> | ||
- | → <a href="https://imm.medicina.ulisboa.pt/intranet/booking/146"><img src="/facility/bioimaging/lib/exe/fetch.php?media=date.png"> Leica SP8 MP Booking</a> | + | → <a href="https://my.agendo.science/calendar/?id=MzRjYWxlbmRhcnM="><img src="/facility/bioimaging/lib/exe/fetch.php?media=date.png"> Leica SP8 MP Booking</a> |
<br> | <br> | ||
→ <a href="/facility/bioimaging/doku.php?id=leica_sp8_mp_quality"><img src="/facility/bioimaging/lib/exe/fetch.php?media=list-icon.png" width=18> Leica SP8 MP Quality Control</a> | → <a href="/facility/bioimaging/doku.php?id=leica_sp8_mp_quality"><img src="/facility/bioimaging/lib/exe/fetch.php?media=list-icon.png" width=18> Leica SP8 MP Quality Control</a> | ||
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^ Laser Unit ^ Wavelength ^ Maximum Power ^ Current Status ^ | ^ Laser Unit ^ Wavelength ^ Maximum Power ^ Current Status ^ | ||
| InSight DS+ Dual (multi-photon) | 680 - 1300 nm | 1.3 W | ok | | | InSight DS+ Dual (multi-photon) | 680 - 1300 nm | 1.3 W | ok | | ||
- | | ::: | 1040 nm | 1.5 W | **not working** | | + | | ::: | 1040 nm | 1.5 W | ok | |
| SS OBIS 488-20 (confocal) | 488 nm | 20 mW | ok | | | SS OBIS 488-20 (confocal) | 488 nm | 20 mW | ok | | ||
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==== Filtersets (Ocular) ==== | ==== Filtersets (Ocular) ==== | ||
- | ^ Filterset ^ Reference ^ Excitation ^ Emission ^ Fluorochromes ^ | + | ^ Filterset ^ Reference ^ Excitation ^ Dichroic ^ Emission ^ Fluorochromes ^ |
- | | Blue | I3 | 450-490 nm | > 515 nm | GFP, FITC, Alexa488 | | + | | Blue | I3 | 450-490 nm | 510 nm | > 515 nm | GFP, FITC, Alexa488 | |
==== Emission Filters (multi-photon) ==== | ==== Emission Filters (multi-photon) ==== | ||
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Else: | Else: | ||
* Clean up immersion objectives | * Clean up immersion objectives | ||
- | * Set the infrared laser to ''standby'' (deactivate the infrared laser in the Currently Available Lasers window) | + | * Set the infrared laser to ''hibernate'' (deactivate the infrared laser in the Currently Available Lasers window) |
* Close the **LAS X** software | * Close the **LAS X** software | ||
* Turn of the 488 nm laser with the key switch in the flexible supply unit | * Turn of the 488 nm laser with the key switch in the flexible supply unit | ||
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- | [[resources|Back to the Resources page]] | + | [[resources|Back to the Equipment page]] |