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leica_sp8_mp [2020/03/01 16:00] bioimaging [Objectives] |
leica_sp8_mp [2020/03/01 17:50] bioimaging [Filtersets (Ocular)] |
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==== Filtersets (Ocular) ==== | ==== Filtersets (Ocular) ==== | ||
- | ^ Filterset ^ Reference ^ Excitation ^ Emission ^ Fluorochromes ^ | + | ^ Filterset ^ Reference ^ Excitation ^ Dichroic ^ Emission ^ Fluorochromes ^ |
- | | Blue | I3 | 450-490 nm | > 515 nm | GFP, FITC, Alexa488 | | + | | Blue | I3 | 450-490 nm | 510 nm | > 515 nm | GFP, FITC, Alexa488 | |
==== Emission Filters (multi-photon) ==== | ==== Emission Filters (multi-photon) ==== | ||
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Else: | Else: | ||
* Clean up immersion objectives | * Clean up immersion objectives | ||
- | * Set the infrared laser to ''standby'' (deactivate the infrared laser in the Currently Available Lasers window) | + | * Set the infrared laser to ''hibernate'' (deactivate the infrared laser in the Currently Available Lasers window) |
* Close the **LAS X** software | * Close the **LAS X** software | ||
* Turn of the 488 nm laser with the key switch in the flexible supply unit | * Turn of the 488 nm laser with the key switch in the flexible supply unit |