leica_sp8_mp
Differences
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| Both sides previous revision Previous revision Next revision | Previous revision Next revision Both sides next revision | ||
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leica_sp8_mp [2020/01/20 16:50] bioimaging [System Turn On Procedures] |
leica_sp8_mp [2020/03/01 16:01] bioimaging [Microscope Turn Off procedures] |
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| ==== LASERs ==== | ==== LASERs ==== | ||
| ^ Laser Unit ^ Wavelength ^ Maximum Power ^ Current Status ^ | ^ Laser Unit ^ Wavelength ^ Maximum Power ^ Current Status ^ | ||
| - | | InSight DS+ Dual (multi-photon) | 680 - 1300 nm | 1.3 W | **not working** | | + | | InSight DS+ Dual (multi-photon) | 680 - 1300 nm | 1.3 W | ok | |
| - | | ::: | 1041 nm | 1.5 W | **not working** | | + | | ::: | 1040 nm | 1.5 W | **not working** | |
| | SS OBIS 488-20 (confocal) | 488 nm | 20 mW | ok | | | SS OBIS 488-20 (confocal) | 488 nm | 20 mW | ok | | ||
| ==== Objectives ==== | ==== Objectives ==== | ||
| ^ Magnification ^ Model ^ Immersion ^ NA ^ WD (mm) ^ Reference ^ | ^ Magnification ^ Model ^ Immersion ^ NA ^ WD (mm) ^ Reference ^ | ||
| - | | 25x | HC FLUOTAR VISIR | Water | 0.95 | 2.5 | 15506374 | | + | | 25x | HC FLUOTAR VISIR | Water | 0.95 | 2.50 | 15506374 | |
| | 63x | HC PL APO Glyc CORR CS2 | Glycerol | 1.30 | 0.30 | 15506353 | | | 63x | HC PL APO Glyc CORR CS2 | Glycerol | 1.30 | 0.30 | 15506353 | | ||
| Line 109: | Line 109: | ||
| Else: | Else: | ||
| * Clean up immersion objectives | * Clean up immersion objectives | ||
| - | * Set the infrared laser to ''standby'' (deactivate the infrared laser in the Currently Available Lasers window) | + | * Set the infrared laser to ''hibernate'' (deactivate the infrared laser in the Currently Available Lasers window) |
| * Close the **LAS X** software | * Close the **LAS X** software | ||
| * Turn of the 488 nm laser with the key switch in the flexible supply unit | * Turn of the 488 nm laser with the key switch in the flexible supply unit | ||
leica_sp8_mp.txt · Last modified: 2024/01/28 12:05 by bioimaging
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