leica_sp8_mp
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leica_sp8_mp [2020/01/17 14:43] bioimaging [Emission Filters (multi-photon)] |
leica_sp8_mp [2020/01/20 16:44] bioimaging [System Turn On Procedures] |
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| ==== Emission Filters (multi-photon) ==== | ==== Emission Filters (multi-photon) ==== | ||
| + | {{::default_configuration.png |}} | ||
| + | \\ | ||
| + | \\ | ||
| + | \\ | ||
| ^ Detector ^ Filter ^ Transmission ^ Fluorochromes ^ | ^ Detector ^ Filter ^ Transmission ^ Fluorochromes ^ | ||
| | HyD-RLD 1 | Red | 570-640 nm | mCherry, Alexa568, Cy3 | | | HyD-RLD 1 | Red | 570-640 nm | mCherry, Alexa568, Cy3 | | ||
| Line 60: | Line 64: | ||
| | PMT-RLD 3 | Far Red | 662 - 737 nm | Alexa647, Cy5 | | | PMT-RLD 3 | Far Red | 662 - 737 nm | Alexa647, Cy5 | | ||
| | PMR-RLD 4 | Red | 570-640 nm | mCherry, Alexa568, Cy3 | | | PMR-RLD 4 | Red | 570-640 nm | mCherry, Alexa568, Cy3 | | ||
| - | + | \\ | |
| - | {{::default_configuration.png|}} | + | \\ |
| + | \\ | ||
| + | \\ | ||
| + | \\ | ||
| **Upon request:** | **Upon request:** | ||
| - | (requires changing the dichroic beamsplitter) | + | \\ (requires changing filters and the dichroic beamsplitter) |
| + | {{::alternative_configuration.png |}} | ||
| + | \\ | ||
| + | \\ | ||
| + | \\ | ||
| ^ Detector ^ Filter ^ Transmission ^ Fluorochromes ^ | ^ Detector ^ Filter ^ Transmission ^ Fluorochromes ^ | ||
| | HyD-RLD 1 | Green | 500-550 nm | GFP, Alexa488, FITC | | | HyD-RLD 1 | Green | 500-550 nm | GFP, Alexa488, FITC | | ||
| Line 71: | Line 81: | ||
| | PMT-RLD 3 | Far Red | 662 - 737 nm | Alexa647, Cy5 | | | PMT-RLD 3 | Far Red | 662 - 737 nm | Alexa647, Cy5 | | ||
| | PMR-RLD 4 | Red | 570-640 nm | mCherry, Alexa568, Cy3 | | | PMR-RLD 4 | Red | 570-640 nm | mCherry, Alexa568, Cy3 | | ||
| + | \\ | ||
| + | \\ | ||
| + | \\ | ||
| - | {{::alternative_configuration.png|}} | + | ===== System Turn On Procedures ===== |
| + | * Check that the cooling and power supply for the infrared laser are ON (''InSight DeepSee READY'') | ||
| + | {{::0_infrared_laser.png?100|}} | ||
| + | * Turn on the fluorescence lamp | ||
| + | {{1_metal_halide.png?100|}} | ||
| + | * Turn on the HyD (Hybrid detectors) supply unit | ||
| + | {{::2_hyd_supply.png?100|}} | ||
| + | * Turn on the computer | ||
| + | * Turn on the microscope electronics box | ||
| + | {{::3_electronics_box.png?100|}} | ||
| + | * Turn on the microscope's control panel | ||
| + | {{::4_control_panel.png?150|}} | ||
| + | * Switch on the scanner power, laser power and activate the key switch to turn on the 488 nm laser | ||
| + | {{::5_scanners_laser.png?120|}} | ||
| + | * Log in to Windows (Bioimaging User) | ||
| + | * Start the **LAS X** software | ||
| ---- | ---- | ||
| [[resources|Back to the Resources page]] | [[resources|Back to the Resources page]] | ||
leica_sp8_mp.txt · Last modified: 2024/01/28 12:05 by bioimaging
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