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leica_sp8_mp [2020/01/17 13:10] bioimaging |
leica_sp8_mp [2020/02/28 00:36] bioimaging [LASERs] |
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==== LASERs ==== | ==== LASERs ==== | ||
^ Laser Unit ^ Wavelength ^ Maximum Power ^ Current Status ^ | ^ Laser Unit ^ Wavelength ^ Maximum Power ^ Current Status ^ | ||
- | | InSight DS+ Dual (multi-photon) | 680 - 1300 nm | 1.3 W | **not working** | | + | | InSight DS+ Dual (multi-photon) | 680 - 1300 nm | 1.3 W | ok | |
- | | ::: | 1041 nm | 1.5 W | **not working** | | + | | ::: | 1040 nm | 1.5 W | **not working** | |
| SS OBIS 488-20 (confocal) | 488 nm | 20 mW | ok | | | SS OBIS 488-20 (confocal) | 488 nm | 20 mW | ok | | ||
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==== Emission Filters (multi-photon) ==== | ==== Emission Filters (multi-photon) ==== | ||
+ | {{::default_configuration.png |}} | ||
+ | \\ | ||
+ | \\ | ||
+ | \\ | ||
+ | ^ Detector ^ Filter ^ Transmission ^ Fluorochromes ^ | ||
+ | | HyD-RLD 1 | Red | 570-640 nm | mCherry, Alexa568, Cy3 | | ||
+ | | HyD-RLD 2 | Green | 500-550 nm | GFP, Alexa488, FITC | | ||
+ | | PMT-RLD 3 | Far Red | 662 - 737 nm | Alexa647, Cy5 | | ||
+ | | PMR-RLD 4 | Red | 570-640 nm | mCherry, Alexa568, Cy3 | | ||
+ | \\ | ||
+ | \\ | ||
+ | \\ | ||
+ | \\ | ||
+ | \\ | ||
+ | **Upon request:** | ||
+ | \\ (requires changing filters and the dichroic beamsplitter) | ||
+ | |||
+ | {{::alternative_configuration.png |}} | ||
+ | \\ | ||
+ | \\ | ||
+ | \\ | ||
+ | ^ Detector ^ Filter ^ Transmission ^ Fluorochromes ^ | ||
+ | | HyD-RLD 1 | Green | 500-550 nm | GFP, Alexa488, FITC | | ||
+ | | HyD-RLD 2 | Blue | 415-485 nm | DAPI, Hoescht, Alexa350 | | ||
+ | | PMT-RLD 3 | Far Red | 662 - 737 nm | Alexa647, Cy5 | | ||
+ | | PMR-RLD 4 | Red | 570-640 nm | mCherry, Alexa568, Cy3 | | ||
+ | \\ | ||
+ | \\ | ||
+ | \\ | ||
+ | |||
+ | ===== System Turn On Procedures ===== | ||
+ | * Check that the cooling and power supply for the infrared laser are ON (''InSight DeepSee READY'') | ||
+ | {{::0_infrared_laser.png?100|}} | ||
+ | * Turn on the fluorescence lamp | ||
+ | {{1_metal_halide.png?100|}} | ||
+ | * Turn on the HyD (Hybrid detectors) supply unit | ||
+ | {{::2_hyd_supply.png?100|}} | ||
+ | * Turn on the computer | ||
+ | * Turn on the microscope electronics box | ||
+ | {{::3_electronics_box.png?100|}} | ||
+ | * Turn on the microscope's control panel | ||
+ | {{::4_control_panel.png?150|}} | ||
+ | * Switch on the scanner power, laser power and activate the key switch to turn on the 488 nm laser in the flexible supply unit | ||
+ | {{::5_scanners_laser.png?120|}} | ||
+ | * Log in to Windows (Bioimaging User) | ||
+ | * Start the **LAS X** software | ||
+ | |||
+ | ===== Microscope Turn Off procedures ===== | ||
+ | |||
+ | If there is another user for this microscope in the __next hour__: | ||
+ | * Leave the fluorescent lamp and lasers on | ||
+ | * Clean up immersion objectives | ||
+ | Else: | ||
+ | * Clean up immersion objectives | ||
+ | * Set the infrared laser to ''standby'' (deactivate the infrared laser in the Currently Available Lasers window) | ||
+ | * Close the **LAS X** software | ||
+ | * Turn of the 488 nm laser with the key switch in the flexible supply unit | ||
+ | * Switch off the HyD supply unit | ||
+ | * Switch off the microscope's control panel | ||
+ | * Shutdown the computer | ||
+ | * Switch off the laser power and scanner power in the flexible supply unit | ||
+ | * Turn off the microscope electronics box | ||
+ | * Turn off the fluorescent lamp | ||
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