leica_sp8_mp
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leica_sp8_mp [2020/01/17 13:10] bioimaging |
leica_sp8_mp [2020/01/20 10:46] bioimaging [Emission Filters (multi-photon)] |
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| ==== Emission Filters (multi-photon) ==== | ==== Emission Filters (multi-photon) ==== | ||
| + | {{::default_configuration.png |}} | ||
| + | \\ | ||
| + | \\ | ||
| + | \\ | ||
| + | ^ Detector ^ Filter ^ Transmission ^ Fluorochromes ^ | ||
| + | | HyD-RLD 1 | Red | 570-640 nm | mCherry, Alexa568, Cy3 | | ||
| + | | HyD-RLD 2 | Green | 500-550 nm | GFP, Alexa488, FITC | | ||
| + | | PMT-RLD 3 | Far Red | 662 - 737 nm | Alexa647, Cy5 | | ||
| + | | PMR-RLD 4 | Red | 570-640 nm | mCherry, Alexa568, Cy3 | | ||
| + | \\ | ||
| + | \\ | ||
| + | \\ | ||
| + | \\ | ||
| + | \\ | ||
| + | **Upon request:** | ||
| + | \\ (requires changing filters and the dichroic beamsplitter) | ||
| + | |||
| + | {{::alternative_configuration.png |}} | ||
| + | \\ | ||
| + | \\ | ||
| + | \\ | ||
| + | ^ Detector ^ Filter ^ Transmission ^ Fluorochromes ^ | ||
| + | | HyD-RLD 1 | Green | 500-550 nm | GFP, Alexa488, FITC | | ||
| + | | HyD-RLD 2 | Blue | 415-485 nm | DAPI, Hoescht, Alexa350 | | ||
| + | | PMT-RLD 3 | Far Red | 662 - 737 nm | Alexa647, Cy5 | | ||
| + | | PMR-RLD 4 | Red | 570-640 nm | mCherry, Alexa568, Cy3 | | ||
| + | \\ | ||
| + | \\ | ||
| + | \\ | ||
| + | |||
| + | ===== System Turn On Procedures ===== | ||
| + | * Turn on the fluorescent light power source | ||
| + | {{1_metal_halide.png?100|}} | ||
| ---- | ---- | ||
| [[resources|Back to the Resources page]] | [[resources|Back to the Resources page]] | ||
leica_sp8_mp.txt ยท Last modified: 2024/01/28 12:05 by bioimaging
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