leica_sp8_mp
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leica_sp8_mp [2020/01/17 13:10] bioimaging |
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| <a href="/facility/bioimaging/lib/exe/fetch.php?media=leica_sp8_mp_wiki_new.png"><img src="/facility/bioimaging/lib/exe/fetch.php?media=leica_sp8_mp_wiki_new.png" width=300></a> | <a href="/facility/bioimaging/lib/exe/fetch.php?media=leica_sp8_mp_wiki_new.png"><img src="/facility/bioimaging/lib/exe/fetch.php?media=leica_sp8_mp_wiki_new.png" width=300></a> | ||
| <td style="border:0px solid white;"> <p style="line-height:1.8"> | <td style="border:0px solid white;"> <p style="line-height:1.8"> | ||
| - | <b>Location</b>: Room P2-B-42 (<img src="/facility/bioimaging/lib/exe/fetch.php?media=telephone_icon.gif" width=15> 47246) <br> | + | <b>Location</b>: <a href="/facility/bioimaging/lib/exe/fetch.php?media=leica_sp8_mp_map_new.png">Room P2-B-42</a> (<img src="/facility/bioimaging/lib/exe/fetch.php?media=phone_neg.png" width=15> 47246) <br> |
| <b>Manufacturer</b>: <a href="https://www.leica-microsystems.com/" target="_blank">Leica Microsystems</a><br> | <b>Manufacturer</b>: <a href="https://www.leica-microsystems.com/" target="_blank">Leica Microsystems</a><br> | ||
| - | <b>Model</b>: TCS SP8 MP<br> | + | <b>Model</b>: <a href="https://www.leica-microsystems.com/products/confocal-microscopes/p/leica-tcs-sp8-mp/" target="_blank">TCS SP8 MP</a><br> |
| <b>Nickname</b>: "Multiphoton"<br> | <b>Nickname</b>: "Multiphoton"<br> | ||
| <b>Software</b>: LAS X<br> | <b>Software</b>: LAS X<br> | ||
| + | <b>Software version</b>: 3.5.5.19976<br> | ||
| <b>Year</b>: 2017 <br> | <b>Year</b>: 2017 <br> | ||
| <b>SN</b>: 8100001758 <br> | <b>SN</b>: 8100001758 <br> | ||
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|   <br> |   <br> | ||
|   <br> |   <br> | ||
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| </table> | </table> | ||
| </html> | </html> | ||
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| + | ===== Microscope overview ===== | ||
| + | |||
| + | {{ :confocal_vs_2photon.png?0x300}} The Leica SP8 MP is a both a confocal and //multi-photon microscope// able to generate high-resolution three-dimensional images of thick specimens. Multi-photon excitation (most commonly two photon excitation) is particularly advantageous for imaging thicker samples. Rather than exciting the fluorophore with one photon, multi-photon excitation is produced by two or more lower energy photons which can penetrate deeper in the sample. Moreover, contrarily to confocal, the multi-photon excitation light will only achieve sufficient intensity to cause fluorescence in a specific region. Because of this, no pinhole is needed in multi-photon microscopy to exclude the light from out-of-focus planes and achieve optical sectioning. The Leica SP8 MP is an upright microscope equipped with water and glycerol immersion objectives especially suitable for intra-vital and thick samples immersed in water or glycerol imaging. In confocal mode, its scanning unit includes a spectral detector PMT to be used with a 488 nm laser for excitation. In multi-photon mode, its Insight DS+ Dual pulsed laser can be tuned from 680 to 1300 nm and has a second laser line emitting at 1041 nm, to be used with four non-descanned detectors with specific filtersets: two PMTS and two HyD detectors (hybrid detectors with 45% QE compared to ~25% QE for conventional PMT). With this system you can perform optical sectioning high resolution imaging of fluorescent samples that are two thick for confocal microscopes such as the [[zeiss_lsm_880|Zeiss LSM 880]] or the [[zeiss_lsm_710|Zeiss LSM 710]], albeit with a slightly lower resolution. If you do not need optical sectioning and your sample is thin, check out a widefield system instead, such as the [[zeiss_cell_observer|Cell Observer]] or the [[nikon_eclipse_ti|Nikon Eclipse Ti]]. If your personal computer cannot handle all the data you collected, check out the [[big_guy|Big Guy]] or [[colossus|Colossus]]. | ||
| + | |||
| + | {{:info.png?25|}} Click on the image on the right to see it in higher detail | ||
| + | |||
| + | {{:warning.png?25|}} Data files older than **3 months** will be automatically deleted on this system, please copy your data to the iMM server using the desktop link. | ||
| + | |||
| + | ===== System components ===== | ||
| + | |||
| + | ==== LASERs ==== | ||
| + | ^ Laser Unit ^ Wavelength ^ Maximum Power ^ Current Status ^ | ||
| + | | InSight DS+ Dual (multi-photon) | 680 - 1300 nm | 1.3 W | **not working** | | ||
| + | | ::: | 1041 nm | 1.5 W | **not working** | | ||
| + | | SS OBIS 488-20 (confocal) | 488 nm | 20 mW | ok | | ||
| + | |||
| + | ==== Objectives ==== | ||
| + | ^ Magnification ^ Model ^ Immersion ^ NA ^ WD (mm) ^ Reference ^ | ||
| + | | 25x | HC FLUOTAR VISIR | Water | 0.95 | 2.5 | 15506374 | | ||
| + | | 63x | HC PL APO Glyc CORR CS2 | Glycerol | 1.30 | 0.30 | 15506353 | | ||
| + | |||
| + | ==== Filtersets (Ocular) ==== | ||
| + | |||
| + | ^ Filterset ^ Reference ^ Excitation ^ Emission ^ Fluorochromes ^ | ||
| + | | Blue | I3 | 450-490 nm | > 515 nm | GFP, FITC, Alexa488 | | ||
| + | |||
| + | ==== Emission Filters (multi-photon) ==== | ||
| + | |||
| ---- | ---- | ||
| [[resources|Back to the Resources page]] | [[resources|Back to the Resources page]] | ||
leica_sp8_mp.txt ยท Last modified: 2024/01/28 12:05 by bioimaging
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