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leica_dm5000b [2021/04/06 00:04] bioimaging |
leica_dm5000b [2022/08/30 12:35] bioimaging [Objectives] |
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<a href="/facility/bioimaging/lib/exe/fetch.php?media=leica_dm5000b_wiki_new.png"><img src="/facility/bioimaging/lib/exe/fetch.php?media=leica_dm5000b_wiki_new.png" width=300></a> | <a href="/facility/bioimaging/lib/exe/fetch.php?media=leica_dm5000b_wiki_new.png"><img src="/facility/bioimaging/lib/exe/fetch.php?media=leica_dm5000b_wiki_new.png" width=300></a> | ||
<td style="border:0px solid white;"> <p style="line-height:1.8"> | <td style="border:0px solid white;"> <p style="line-height:1.8"> | ||
- | <b>Location</b>: <a href="/facility/bioimaging/lib/exe/fetch.php?media=leica_dm5000b_map_new.png">Room P0-B-32</a><br> | + | <b>Location</b>: <a href="/facility/bioimaging/lib/exe/fetch.php?media=leica_dm5000b_map_new.png">Room P2-A-15</a><br> |
<b>Manufacturer</b>: <a href="http://www.leica-microsystems.com/home/" target="_blank">Leica Microsystems</a><br> | <b>Manufacturer</b>: <a href="http://www.leica-microsystems.com/home/" target="_blank">Leica Microsystems</a><br> | ||
<b>Model</b>: <a href="http://www.leica-microsystems.com/products/light-microscopes/life-science-research/upright-microscopes/details/product/leica-dm5000-b/" target="_blank">DM5000 B</a><br> | <b>Model</b>: <a href="http://www.leica-microsystems.com/products/light-microscopes/life-science-research/upright-microscopes/details/product/leica-dm5000-b/" target="_blank">DM5000 B</a><br> | ||
<b>Nickname</b>: "5000B"<br> | <b>Nickname</b>: "5000B"<br> | ||
- | <b>Software</b>: Leica DC Twain<br> | ||
<b>Year</b>: 2006 <br> | <b>Year</b>: 2006 <br> | ||
<b>SN</b>: 269835<br> | <b>SN</b>: 269835<br> | ||
+ |   <br> | ||
  <br> |   <br> | ||
  <br> |   <br> | ||
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===== Microscope overview ===== | ===== Microscope overview ===== | ||
- | The Leica DM5000B is an automated upright //widefield fluorescence microscope// ideal for imaging FISH or other fixed fluorescence slides. The microscope is prepared for brightfield, phase contrast, DIC and polarization contrast techniques (check objectives list for contrast method availability). It has a monochrome CCD camera for fluorescence image acquisition. If you want to image live samples and/or perform time-lapse experiments check out the [[zeiss_axiovert_200m|Zeiss Axiovert 200M]] or the [[zeiss_cell_observer|Zeiss Cell Observer]] instead. If you need a color camera for brightfield images, try the [[leica_dm2500|Leica DM2500]]. | + | The Leica DM5000B is an automated upright //widefield fluorescence microscope// ideal for checking FISH or other fixed fluorescence slides. The microscope is prepared for brightfield, phase contrast, DIC and polarization contrast techniques (check objectives list for contrast method availability). If you want to image live samples and/or perform time-lapse experiments check out the [[zeiss_axiovert_200m|Zeiss Axiovert 200M]] or the [[zeiss_cell_observer|Zeiss Cell Observer]] instead. If you need a color camera for brightfield images, try the [[leica_dm2500|Leica DM2500]]. |
- | + | ||
- | {{:warning.png?25|}} Data files older than **3 months** will be automatically deleted on this system, please copy your data to the iMM server using the desktop link. | + | |
===== System components ===== | ===== System components ===== | ||
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==== Objectives ==== | ==== Objectives ==== | ||
- | ^ Magnification ^ Model ^ Immersion ^ NA ^ WD (mm) ^ pixel size* (μm) ^ 10 μm = ^ BF ^ PH ^ DIC ^ POL ^ Reference ^ | + | ^ Magnification ^ Model ^ Immersion ^ NA ^ WD (mm) ^ BF ^ PH ^ DIC ^ POL ^ Reference ^ |
- | | 5x | HC PL FLUOTAR | Air | 0.15 | 12.00 | 2.00 | 5 pixels | ✔ | ✘ | ✘ | ✔ | 506504 | | + | | 5x | HC PL FLUOTAR | Air | 0.15 | 12.00 | ✔ | ✘ | ✘ | ✔ | 506504 | |
- | | 10x | HC PL FLUOTAR | Air | 0.30 | 11.00 | 1.00 | 10 pixels | ✔ | ✔ | ✘ | ✔ | 506507 | | + | | 10x | HC PL FLUOTAR | Air | 0.30 | 11.00 | ✔ | ✔ | ✘ | ✔ | 506507 | |
- | | 20x | HC PL FLUOTAR | Air | 0.50 | 1.15 | 0.53 | 19 pixels | ✔ | ✔ | ✔ | ✔ | 506506 | | + | | 20x | HC PL FLUOTAR | Air | 0.50 | 1.15 | ✔ | ✔ | ✔ | ✔ | 506506 | |
- | | 20x | HC PLAN APO | Air | 0.70 | 0.59 | 0.53 | 19 pixels | ✔ | ✘ | ✔ | ✔ | 506166 | | + | | 20x | HC PLAN APO | Air | 0.70 | 0.59 | ✔ | ✘ | ✔ | ✔ | 506166 | |
- | | 40x | HCX PL FLUOTAR | Air | 0.75 | 0.40 | 0.26 | 39 pixels | ✔ | ✔ | ✔ | ✔ | 506145 | | + | | 40x | HCX PL FLUOTAR | Air | 0.75 | 0.40 | ✔ | ✔ | ✔ | ✔ | 506145 | |
- | | 63x | HCX PL APO | Oil | 1.4-0.60 | 0.10 | 0.16 | 63 pixels | ✔ | ✘ | ✔ | ✔ | 506187 | | + | | 63x | HCX PL APO | Oil | 1.4-0.60 | 0.10 | ✔ | ✘ | ✔ | ✔ | 506187 | |
- | + | ||
- | *** pixel size for 1300 x 1030 images** | + | |
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[[resources|Back to the Equipment page]] | [[resources|Back to the Equipment page]] |