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leica_dm5000b [2020/01/17 12:35] bioimaging [Microscope overview] |
leica_dm5000b [2021/04/06 00:04] bioimaging |
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  <br> |   <br> | ||
- |   <br> | + | → <a href="https://my.agendo.science/calendar/?id=MzJjYWxlbmRhcnM="><img src="/facility/bioimaging/lib/exe/fetch.php?media=date.png"> Leica DM5000B Booking</a> |
- | → <a href="https://imm.medicina.ulisboa.pt/intranet/booking/9"><img src="/facility/bioimaging/lib/exe/fetch.php?media=date.png"> Leica DM5000B Booking</a> | + | <br> |
- | <br> | + | → <a href="/facility/bioimaging/doku.php?id=leica_dm5000b_quality"><img src="/facility/bioimaging/lib/exe/fetch.php?media=list-icon.png" width=18> Leica DM5000B Quality Control</a> |
+ | <br> | ||
→ <a href="/facility/bioimaging/doku.php?id=leica_dm5000b_usage"><img src="/facility/bioimaging/lib/exe/fetch.php?media=chart_line.png"> Leica DM5000B Usage Statistics</a> | → <a href="/facility/bioimaging/doku.php?id=leica_dm5000b_usage"><img src="/facility/bioimaging/lib/exe/fetch.php?media=chart_line.png"> Leica DM5000B Usage Statistics</a> | ||
</p> | </p> | ||
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==== Filtersets ==== | ==== Filtersets ==== | ||
- | ^ Filterset ^ Reference ^ Excitation ^ Emission ^ Fluorochromes ^ | + | ^ Filterset ^ Reference ^ Excitation ^ Dichroic ^ Emission ^ Fluorochromes ^ |
- | | Blue | A4 | 340-380 nm | 450-490 nm | DAPI, Hoechst, Alexa350 | | + | | Blue | A4 | 340-380 nm | 400 nm | 450-490 nm | DAPI, Hoechst, Alexa350 | |
- | | Green | GFP | 450-490 nm | 500-550 nm | GFP, Cy2, FITC | | + | | Green | GFP | 450-490 nm | 500 nm | 500-550 nm | GFP, FITC, Alexa488 | |
- | | Red | N2.1 | 515-560 nm | > 590 nm | Rhodamine, Cy3, Texas Red | | + | | Red | N2.1 | 515-560 nm | 580 nm | > 590 nm | Rhodamine, Cy3, Texas Red | |
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==== Objectives ==== | ==== Objectives ==== | ||
- | ^ Magnification ^ Model ^ Immersion ^ NA ^ WD (mm) ^ pixel size* (μm) ^ 10 μm = ^ ^ BF ^ PH ^ DIC ^ POL ^ | + | ^ Magnification ^ Model ^ Immersion ^ NA ^ WD (mm) ^ pixel size* (μm) ^ 10 μm = ^ BF ^ PH ^ DIC ^ POL ^ Reference ^ |
- | | 5x | HC PL FLUOTAR | Air | 0.15 | 12 | 2.00 | 5 pixels | | Yes | No | No | Yes | | + | | 5x | HC PL FLUOTAR | Air | 0.15 | 12.00 | 2.00 | 5 pixels | ✔ | ✘ | ✘ | ✔ | 506504 | |
- | | 10x | HC PL FLUOTAR | Air | 0.30 | 11 | 1.00 | 10 pixels | | Yes | Yes | No | Yes | | + | | 10x | HC PL FLUOTAR | Air | 0.30 | 11.00 | 1.00 | 10 pixels | ✔ | ✔ | ✘ | ✔ | 506507 | |
- | | 20x | HC PL FLUOTAR | Air | 0.50 | 1.15 | 0.53 | 19 pixels | | Yes | Yes | Yes | Yes | | + | | 20x | HC PL FLUOTAR | Air | 0.50 | 1.15 | 0.53 | 19 pixels | ✔ | ✔ | ✔ | ✔ | 506506 | |
- | | 20x | HC PLAN APO | Air | 0.70 | 0.59 | 0.53 | 19 pixels | | Yes | No | Yes | Yes | | + | | 20x | HC PLAN APO | Air | 0.70 | 0.59 | 0.53 | 19 pixels | ✔ | ✘ | ✔ | ✔ | 506166 | |
- | | 40x | HCX PL FLUOTAR | Air | 0.75 | 0.40 | 0.26 | 39 pixels | | Yes | Yes | Yes | Yes | | + | | 40x | HCX PL FLUOTAR | Air | 0.75 | 0.40 | 0.26 | 39 pixels | ✔ | ✔ | ✔ | ✔ | 506145 | |
- | | 63x | HCX PL APO | Oil | 1.4-0.60 | 0.1 | 0.16 | 63 pixels | | Yes | No | Yes | Yes | | + | | 63x | HCX PL APO | Oil | 1.4-0.60 | 0.10 | 0.16 | 63 pixels | ✔ | ✘ | ✔ | ✔ | 506187 | |
*** pixel size for 1300 x 1030 images** | *** pixel size for 1300 x 1030 images** | ||
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- | **Upon request:** | ||
- | |||
- | ^ Magnification ^ Model ^ Type ^ NA ^ WD (mm) ^ pixel size* (μm) ^ 10 μm = ^ BF ^ PH ^ DIC ^ POL ^ | ||
- | | 100x | HCX PL FLUOTAR | Oil | 1.3 | 0.13 | 0.10 | 96 pixels | Yes | No | Yes | Yes | | ||
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- | [[resources|Back to the Resources page]] | + | [[resources|Back to the Equipment page]] |