This shows you the differences between two versions of the page.
Both sides previous revision Previous revision Next revision | Previous revision Next revision Both sides next revision | ||
leica_dm5000b [2020/01/17 12:35] bioimaging [Microscope overview] |
leica_dm5000b [2020/03/01 11:50] bioimaging [Objectives] |
||
---|---|---|---|
Line 38: | Line 38: | ||
^ Filterset ^ Reference ^ Excitation ^ Emission ^ Fluorochromes ^ | ^ Filterset ^ Reference ^ Excitation ^ Emission ^ Fluorochromes ^ | ||
| Blue | A4 | 340-380 nm | 450-490 nm | DAPI, Hoechst, Alexa350 | | | Blue | A4 | 340-380 nm | 450-490 nm | DAPI, Hoechst, Alexa350 | | ||
- | | Green | GFP | 450-490 nm | 500-550 nm | GFP, Cy2, FITC | | + | | Green | GFP | 450-490 nm | 500-550 nm | GFP, FITC, Alexa488 | |
| Red | N2.1 | 515-560 nm | > 590 nm | Rhodamine, Cy3, Texas Red | | | Red | N2.1 | 515-560 nm | > 590 nm | Rhodamine, Cy3, Texas Red | | ||
Line 45: | Line 45: | ||
==== Objectives ==== | ==== Objectives ==== | ||
- | ^ Magnification ^ Model ^ Immersion ^ NA ^ WD (mm) ^ pixel size* (μm) ^ 10 μm = ^ ^ BF ^ PH ^ DIC ^ POL ^ | + | ^ Magnification ^ Model ^ Immersion ^ NA ^ WD (mm) ^ pixel size* (μm) ^ 10 μm = ^ ^ BF ^ PH ^ DIC ^ POL ^ Reference ^ |
- | | 5x | HC PL FLUOTAR | Air | 0.15 | 12 | 2.00 | 5 pixels | | Yes | No | No | Yes | | + | | 5x | HC PL FLUOTAR | Air | 0.15 | 12.00 | 2.00 | 5 pixels | | ✔ | ✘ | ✘ | ✔ | 506504 | |
- | | 10x | HC PL FLUOTAR | Air | 0.30 | 11 | 1.00 | 10 pixels | | Yes | Yes | No | Yes | | + | | 10x | HC PL FLUOTAR | Air | 0.30 | 11.00 | 1.00 | 10 pixels | | ✔ | ✔ | ✘ | ✔ | 506507 | |
- | | 20x | HC PL FLUOTAR | Air | 0.50 | 1.15 | 0.53 | 19 pixels | | Yes | Yes | Yes | Yes | | + | | 20x | HC PL FLUOTAR | Air | 0.50 | 1.15 | 0.53 | 19 pixels | | ✔ | ✔ | ✔ | ✔ | 506506 | |
- | | 20x | HC PLAN APO | Air | 0.70 | 0.59 | 0.53 | 19 pixels | | Yes | No | Yes | Yes | | + | | 20x | HC PLAN APO | Air | 0.70 | 0.59 | 0.53 | 19 pixels | | ✔ | ✘ | ✔ | ✔ | 506166 | |
- | | 40x | HCX PL FLUOTAR | Air | 0.75 | 0.40 | 0.26 | 39 pixels | | Yes | Yes | Yes | Yes | | + | | 40x | HCX PL FLUOTAR | Air | 0.75 | 0.40 | 0.26 | 39 pixels | | ✔ | ✔ | ✔ | ✔ | 506145 | |
- | | 63x | HCX PL APO | Oil | 1.4-0.60 | 0.1 | 0.16 | 63 pixels | | Yes | No | Yes | Yes | | + | | 63x | HCX PL APO | Oil | 1.4-0.60 | 0.10 | 0.16 | 63 pixels | | ✔ | ✘ | ✔ | ✔ | 506187 | |
*** pixel size for 1300 x 1030 images** | *** pixel size for 1300 x 1030 images** |