leica_dm5000b
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leica_dm5000b [2020/01/01 22:23] bioimaging [Microscope overview] |
leica_dm5000b [2020/01/01 22:29] bioimaging [Objectives] |
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| ^ Filterset ^ Reference ^ Excitation ^ Emission ^ Fluorochromes ^ | ^ Filterset ^ Reference ^ Excitation ^ Emission ^ Fluorochromes ^ | ||
| - | | Blue | A4 | 320-400 nm | 430-510 nm | DAPI, Hoechst, Alexa350 | | + | | Blue | A4 | 340-380 nm | 450-490 nm | DAPI, Hoechst, Alexa350 | |
| | Green | GFP | 450-490 nm | 500-550 nm | GFP, Cy2, FITC | | | Green | GFP | 450-490 nm | 500-550 nm | GFP, Cy2, FITC | | ||
| - | | Red | N21 | 515-560 nm | > 590 nm | Rhodamine, Cy3, Texas Red | | + | | Red | N2.1 | 515-560 nm | > 590 nm | Rhodamine, Cy3, Texas Red | |
| Line 44: | Line 44: | ||
| ==== Objectives ==== | ==== Objectives ==== | ||
| - | ^ Magnification ^ Model ^ Type ^ NA ^ WD (mm) ^ pixel size* (μm) ^ 10 μm = ^ ^ BF ^ PH ^ DIC ^ POL ^ | + | ^ Magnification ^ Model ^ Immersion ^ NA ^ WD (mm) ^ pixel size* (μm) ^ 10 μm = ^ ^ BF ^ PH ^ DIC ^ POL ^ |
| - | | 5x | HC PL FLUOTAR | Dry | 0.15 | 12 | 2.00 | 5 pixels | | Yes | No | No | Yes | | + | | 5x | HC PL FLUOTAR | Air | 0.15 | 12 | 2.00 | 5 pixels | | Yes | No | No | Yes | |
| - | | 10x | HC PL FLUOTAR | Dry | 0.30 | 11 | 1.00 | 10 pixels | | Yes | Yes | No | Yes | | + | | 10x | HC PL FLUOTAR | Air | 0.30 | 11 | 1.00 | 10 pixels | | Yes | Yes | No | Yes | |
| - | | 20x | HC PL FLUOTAR | Dry | 0.50 | 1.15 | 0.53 | 19 pixels | | Yes | Yes | Yes | Yes | | + | | 20x | HC PL FLUOTAR | Air | 0.50 | 1.15 | 0.53 | 19 pixels | | Yes | Yes | Yes | Yes | |
| - | | 20x | HC PLAN APO | Dry | 0.70 | 0.59 | 0.53 | 19 pixels | | Yes | No | Yes | Yes | | + | | 20x | HC PLAN APO | Air | 0.70 | 0.59 | 0.53 | 19 pixels | | Yes | No | Yes | Yes | |
| - | | 40x | HCX PL FLUOTAR | Dry | 0.75 | 0.40 | 0.26 | 39 pixels | | Yes | Yes | Yes | Yes | | + | | 40x | HCX PL FLUOTAR | Air | 0.75 | 0.40 | 0.26 | 39 pixels | | Yes | Yes | Yes | Yes | |
| | 63x | HCX PL APO | Oil | 1.4-0.60 | 0.1 | 0.16 | 63 pixels | | Yes | No | Yes | Yes | | | 63x | HCX PL APO | Oil | 1.4-0.60 | 0.1 | 0.16 | 63 pixels | | Yes | No | Yes | Yes | | ||
leica_dm5000b.txt · Last modified: 2023/08/06 13:30 by bioimaging
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