leica_dm5000b
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leica_dm5000b [2016/04/09 03:32] bioimaging created |
leica_dm5000b [2020/03/01 11:50] bioimaging [Objectives] |
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| - | <img src="/facility/bioimaging/lib/exe/fetch.php?media=leica_dm5000b_name.jpg"> | + | <img src="/facility/bioimaging/lib/exe/fetch.php?media=leica_dm5000b_name_new.png"> |
| <table style="border:0px solid white;"> | <table style="border:0px solid white;"> | ||
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| - | <a href="/facility/bioimaging/lib/exe/fetch.php?media=leica_dm5000b_wiki.jpg"><img src="/facility/bioimaging/lib/exe/fetch.php?media=leica_dm5000b_wiki.jpg" width=300></a> | + | <a href="/facility/bioimaging/lib/exe/fetch.php?media=leica_dm5000b_wiki_new.png"><img src="/facility/bioimaging/lib/exe/fetch.php?media=leica_dm5000b_wiki_new.png" width=300></a> |
| <td style="border:0px solid white;"> <p style="line-height:1.8"> | <td style="border:0px solid white;"> <p style="line-height:1.8"> | ||
| - | <b>Location</b>: <a href="/facility/bioimaging/lib/exe/fetch.php?media=leica_dm5000b_map.jpg">Room P3-A-44</a><br> | + | <b>Location</b>: <a href="/facility/bioimaging/lib/exe/fetch.php?media=leica_dm5000b_map_new.png">Room P0-B-32</a><br> |
| <b>Manufacturer</b>: <a href="http://www.leica-microsystems.com/home/" target="_blank">Leica Microsystems</a><br> | <b>Manufacturer</b>: <a href="http://www.leica-microsystems.com/home/" target="_blank">Leica Microsystems</a><br> | ||
| <b>Model</b>: <a href="http://www.leica-microsystems.com/products/light-microscopes/life-science-research/upright-microscopes/details/product/leica-dm5000-b/" target="_blank">DM5000 B</a><br> | <b>Model</b>: <a href="http://www.leica-microsystems.com/products/light-microscopes/life-science-research/upright-microscopes/details/product/leica-dm5000-b/" target="_blank">DM5000 B</a><br> | ||
| + | <b>Nickname</b>: "5000B"<br> | ||
| <b>Software</b>: Leica DC Twain<br> | <b>Software</b>: Leica DC Twain<br> | ||
| <b>Year</b>: 2006 <br> | <b>Year</b>: 2006 <br> | ||
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| - | → <a href="https://imm.medicina.ulisboa.pt/intranet/booking/month.php?user=AXIOVERT&rid=3"><img src="/facility/bioimaging/lib/exe/fetch.php?media=date.png"> Zeiss Axiovert 200M Booking</a> | + |   <br> |
| + | → <a href="https://imm.medicina.ulisboa.pt/intranet/booking/9"><img src="/facility/bioimaging/lib/exe/fetch.php?media=date.png"> Leica DM5000B Booking</a> | ||
| <br> | <br> | ||
| - | → <a href="/facility/bioimaging/doku.php?id=zeiss_axiovert200m_quality"><img src="/facility/bioimaging/lib/exe/fetch.php?media=list-icon.png" width=18> Zeiss Axiovert 200M Quality Control</a> | + | → <a href="/facility/bioimaging/doku.php?id=leica_dm5000b_usage"><img src="/facility/bioimaging/lib/exe/fetch.php?media=chart_line.png"> Leica DM5000B Usage Statistics</a> |
| - | <br> | + | </p> |
| - | → <a href="/facility/bioimaging/doku.php?id=zeiss_axiovert_200m_usage"><img src="/facility/bioimaging/lib/exe/fetch.php?media=chart_line.png"> Zeiss Axiovert 200M Usage Statistics</a> | + | |
| - | <br> | + | |
| - | → <a href="/facility/bioimaging/doku.php?id=zeiss_axiovert_200m_repair"><img src="/facility/bioimaging/lib/exe/fetch.php?media=repair-icon.gif" width=16> Zeiss Axiovert 200M Repair History</a> | + | |
| - | </p> | + | |
| </td> | </td> | ||
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| + | ===== Microscope overview ===== | ||
| + | |||
| + | The Leica DM5000B is an automated upright //widefield fluorescence microscope// ideal for imaging FISH or other fixed fluorescence slides. The microscope is prepared for brightfield, phase contrast, DIC and polarization contrast techniques (check objectives list for contrast method availability). It has a monochrome CCD camera for fluorescence image acquisition. If you want to image live samples and/or perform time-lapse experiments check out the [[zeiss_axiovert_200m|Zeiss Axiovert 200M]] or the [[zeiss_cell_observer|Zeiss Cell Observer]] instead. If you need a color camera for brightfield images, try the [[leica_dm2500|Leica DM2500]]. | ||
| + | |||
| + | {{:warning.png?25|}} Data files older than **3 months** will be automatically deleted on this system, please copy your data to the iMM server using the desktop link. | ||
| + | |||
| + | ===== System components ===== | ||
| + | |||
| + | ==== Filtersets ==== | ||
| + | |||
| + | ^ Filterset ^ Reference ^ Excitation ^ Emission ^ Fluorochromes ^ | ||
| + | | Blue | A4 | 340-380 nm | 450-490 nm | DAPI, Hoechst, Alexa350 | | ||
| + | | Green | GFP | 450-490 nm | 500-550 nm | GFP, FITC, Alexa488 | | ||
| + | | Red | N2.1 | 515-560 nm | > 590 nm | Rhodamine, Cy3, Texas Red | | ||
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| + | ==== Objectives ==== | ||
| + | ^ Magnification ^ Model ^ Immersion ^ NA ^ WD (mm) ^ pixel size* (μm) ^ 10 μm = ^ ^ BF ^ PH ^ DIC ^ POL ^ Reference ^ | ||
| + | | 5x | HC PL FLUOTAR | Air | 0.15 | 12.00 | 2.00 | 5 pixels | | ✔ | ✘ | ✘ | ✔ | 506504 | | ||
| + | | 10x | HC PL FLUOTAR | Air | 0.30 | 11.00 | 1.00 | 10 pixels | | ✔ | ✔ | ✘ | ✔ | 506507 | | ||
| + | | 20x | HC PL FLUOTAR | Air | 0.50 | 1.15 | 0.53 | 19 pixels | | ✔ | ✔ | ✔ | ✔ | 506506 | | ||
| + | | 20x | HC PLAN APO | Air | 0.70 | 0.59 | 0.53 | 19 pixels | | ✔ | ✘ | ✔ | ✔ | 506166 | | ||
| + | | 40x | HCX PL FLUOTAR | Air | 0.75 | 0.40 | 0.26 | 39 pixels | | ✔ | ✔ | ✔ | ✔ | 506145 | | ||
| + | | 63x | HCX PL APO | Oil | 1.4-0.60 | 0.10 | 0.16 | 63 pixels | | ✔ | ✘ | ✔ | ✔ | 506187 | | ||
| + | |||
| + | *** pixel size for 1300 x 1030 images** | ||
| + | |||
| + | **Upon request:** | ||
| + | |||
| + | ^ Magnification ^ Model ^ Type ^ NA ^ WD (mm) ^ pixel size* (μm) ^ 10 μm = ^ BF ^ PH ^ DIC ^ POL ^ | ||
| + | | 100x | HCX PL FLUOTAR | Oil | 1.3 | 0.13 | 0.10 | 96 pixels | Yes | No | Yes | Yes | | ||
| + | |||
| + | ---- | ||
| + | [[resources|Back to the Resources page]] | ||
leica_dm5000b.txt · Last modified: 2023/08/06 13:30 by bioimaging
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