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leica_dm2500 [2020/01/01 23:26] bioimaging |
leica_dm2500 [2024/03/19 12:36] bioimaging |
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- | <a href="/facility/bioimaging/lib/exe/fetch.php?media=leica_dm2500_wiki_new.png"><img src="/facility/bioimaging/lib/exe/fetch.php?media=leica_dm2500_wiki_new.png" width=300></a> | + | <a href="/facility/bioimaging/lib/exe/fetch.php?media=leica_dm2500_wiki_2022.png"><img src="/facility/bioimaging/lib/exe/fetch.php?media=leica_dm2500_wiki_2022.png" width=300></a> |
<td style="border:0px solid white;"> <p style="line-height:1.8"> | <td style="border:0px solid white;"> <p style="line-height:1.8"> | ||
- | <b>Location</b>: <a href="/facility/bioimaging/lib/exe/fetch.php?media=leica_dm2500_map_new.png">Room P3-A-5</a><br> | + | <b>Location</b>: <a href="/facility/bioimaging/lib/exe/fetch.php?media=leica_dm2500_map_new.png">Room P3-A-16b</a><br> |
<b>Manufacturer</b>: <a href="http://www.leica-microsystems.com/home/" target="_blank">Leica Microsystems</a><br> | <b>Manufacturer</b>: <a href="http://www.leica-microsystems.com/home/" target="_blank">Leica Microsystems</a><br> | ||
<b>Model</b>: DM2500<br> | <b>Model</b>: DM2500<br> | ||
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<b>Year</b>: 2006 <br> | <b>Year</b>: 2006 <br> | ||
<b>SN</b>: 285533-102006<br> | <b>SN</b>: 285533-102006<br> | ||
+ |   <br> | ||
+ | Data will be deleted after: <b>3 months</b> | ||
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- | → <a href="https://imm.medicina.ulisboa.pt/intranet/booking/10"><img src="/facility/bioimaging/lib/exe/fetch.php?media=date.png"> Leica DM2500 Booking</a> | ||
- | <br> | ||
→ <a href="/facility/bioimaging/doku.php?id=leica_dm2500_usage"><img src="/facility/bioimaging/lib/exe/fetch.php?media=chart_line.png"> Leica DM2500 Usage Statistics</a> | → <a href="/facility/bioimaging/doku.php?id=leica_dm2500_usage"><img src="/facility/bioimaging/lib/exe/fetch.php?media=chart_line.png"> Leica DM2500 Usage Statistics</a> | ||
</p> | </p> | ||
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===== Microscope overview ===== | ===== Microscope overview ===== | ||
- | The Leica DM2500 is an upright //brightfield microscope// equipped with a color camera for brightfield and differential interference contrast (DIC) imaging. If you need to perform fluorescence imaging try the [[leica_dm5000b|Leica DM5000B]] instead. | + | The Leica DM2500 is an upright //brightfield microscope// equipped with a color camera ([[https://www.leica-microsystems.com/products/microscope-cameras/p/leica-dfc450/|Leica DFC450]]) for brightfield and differential interference contrast (DIC) imaging. |
- | {{warning.jpg?20|}} Data files older than **3 months** will be automatically deleted on this system, please copy your data to the iMM server using the desktop link. | + | {{:warning.png?25|}} Data files older than **3 months** will be automatically deleted on this system, please copy your data to the iMM server using the desktop link. |
===== System components ===== | ===== System components ===== | ||
==== Objectives ==== | ==== Objectives ==== | ||
- | ^ Magnification ^ Model ^ Type ^ NA ^ WD (mm) ^ pixel size (μm) ^ 10 μm = ^ | + | ^ Magnification ^ Model ^ Type ^ NA ^ WD (mm) ^ pixel size (μm) ^ 10 μm = ^ Reference ^ |
- | | 1.25x | HCX PL FLUOTAR | Dry | 0.04 | 3.70 | 4.9 | 2.02 pixels | | + | | 1.25x | HCX PL FLUOTAR | Dry | 0.04 | 3.70 | 4.90 | 2.0 pixels | 506215 | |
- | | 10x | HC PL FLUOTAR | Dry | 0.30 | 11 | 0.61 | 16.2 pixels | | + | | 5x | N PLAN | Dry | 0.12 | 14.00 | 1.23 | 8.1 pixels | 506302 | |
- | | 20x | HC PL FLUOTAR | Dry | 0.50 | 1.15 | 0.30 | 32.5 pixels | | + | | 10x | HC PL FLUOTAR | Dry | 0.30 | 11.00 | 0.61 | 16.2 pixels | 506507 | |
- | | 40x | HCX PL FLUOTAR | Dry | 0.75 | 0.40 | 0.15 | 65 pixels | | + | | 20x | HC PL FLUOTAR | Dry | 0.50 | 1.15 | 0.30 | 32.5 pixels | 506506 | |
- | | 100x | HCX PL FLUOTAR | Oil | 1.30 | 0.22 | 0.06 | 162.5 pixels | | + | | 40x | HCX PL FLUOTAR | Dry | 0.75 | 0.40 | 0.15 | 65 pixels | 506144 | |
+ | | 100x | HCX PL FLUOTAR | Oil | 1.30 | 0.22 | 0.06 | 162.5 pixels | 506195 | | ||
*** pixel size for 2560 x 1920 images (Full Frame)** | *** pixel size for 2560 x 1920 images (Full Frame)** | ||
- | + | ==== Camera ==== | |
- | + | ^ Model ^ Type ^ Frame Size ^ Pixel Size (µm) ^ | |
- | **Upon request:** | + | | [[https://www.leica-microsystems.com/products/microscope-cameras/p/leica-dfc450-c/|Leica DFC450]] | color CCD | 2560 × 1920 | 3.4 x 3.4 | |
- | + | ||
- | ^ Magnification ^ Model ^ Type ^ NA ^ WD (mm) ^ pixel size (μm) ^ 10 μm = ^ | + | |
- | | 5x | N PLAN | Dry | 0.12 | 14 | 1.23 | 8.1 pixels | | + | |
- | | 63x | HCX PL APO | Oil | 1.32-0.60 | 0.1 | 0.1 | 102 pixels | | + | |
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- | [[resources|Back to the Resources page]] | + | [[resources|Back to the Equipment page]] |