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leica_dm2500 [2020/01/01 23:26] bioimaging |
leica_dm2500 [2020/06/01 19:27] bioimaging |
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- | → <a href="https://imm.medicina.ulisboa.pt/intranet/booking/10"><img src="/facility/bioimaging/lib/exe/fetch.php?media=date.png"> Leica DM2500 Booking</a> | + |   <br> |
+ | → <a href="https://my.agendo.science/calendar/?id=MzNjYWxlbmRhcnM="><img src="/facility/bioimaging/lib/exe/fetch.php?media=date.png"> Leica DM2500 Booking</a> | ||
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→ <a href="/facility/bioimaging/doku.php?id=leica_dm2500_usage"><img src="/facility/bioimaging/lib/exe/fetch.php?media=chart_line.png"> Leica DM2500 Usage Statistics</a> | → <a href="/facility/bioimaging/doku.php?id=leica_dm2500_usage"><img src="/facility/bioimaging/lib/exe/fetch.php?media=chart_line.png"> Leica DM2500 Usage Statistics</a> | ||
- | <br> | + | </p> |
- | → <a href="/facility/bioimaging/doku.php?id=leica_dm2500_repair"><img src="/facility/bioimaging/lib/exe/fetch.php?media=repair-icon.gif" width=16> Leica DM2500 Repair History</a> | + | |
- | </p> | + | |
</td> | </td> | ||
</tr> | </tr> | ||
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===== Microscope overview ===== | ===== Microscope overview ===== | ||
- | The Leica DM2500 is an upright //brightfield microscope// equipped with a color camera for brightfield and differential interference contrast (DIC) imaging. If you need to perform fluorescence imaging try the [[leica_dm5000b|Leica DM5000B]] instead. | + | The Leica DM2500 is an upright //brightfield microscope// equipped with a color camera (Leica DFC450) for brightfield and differential interference contrast (DIC) imaging. If you need to perform fluorescence imaging try the [[leica_dm5000b|Leica DM5000B]] instead. |
- | {{warning.jpg?20|}} Data files older than **3 months** will be automatically deleted on this system, please copy your data to the iMM server using the desktop link. | + | {{:warning.png?25|}} Data files older than **3 months** will be automatically deleted on this system, please copy your data to the iMM server using the desktop link. |
===== System components ===== | ===== System components ===== | ||
==== Objectives ==== | ==== Objectives ==== | ||
- | ^ Magnification ^ Model ^ Type ^ NA ^ WD (mm) ^ pixel size (μm) ^ 10 μm = ^ | + | ^ Magnification ^ Model ^ Type ^ NA ^ WD (mm) ^ pixel size (μm) ^ 10 μm = ^ Reference ^ |
- | | 1.25x | HCX PL FLUOTAR | Dry | 0.04 | 3.70 | 4.9 | 2.02 pixels | | + | | 1.25x | HCX PL FLUOTAR | Dry | 0.04 | 3.70 | 4.90 | 2.0 pixels | 506215 | |
- | | 10x | HC PL FLUOTAR | Dry | 0.30 | 11 | 0.61 | 16.2 pixels | | + | | 5x | N PLAN | Dry | 0.12 | 14.00 | 1.23 | 8.1 pixels | 506302 | |
- | | 20x | HC PL FLUOTAR | Dry | 0.50 | 1.15 | 0.30 | 32.5 pixels | | + | | 10x | HC PL FLUOTAR | Dry | 0.30 | 11.00 | 0.61 | 16.2 pixels | 506507 | |
- | | 40x | HCX PL FLUOTAR | Dry | 0.75 | 0.40 | 0.15 | 65 pixels | | + | | 20x | HC PL FLUOTAR | Dry | 0.50 | 1.15 | 0.30 | 32.5 pixels | 506506 | |
- | | 100x | HCX PL FLUOTAR | Oil | 1.30 | 0.22 | 0.06 | 162.5 pixels | | + | | 40x | HCX PL FLUOTAR | Dry | 0.75 | 0.40 | 0.15 | 65 pixels | 506144 | |
+ | | 100x | HCX PL FLUOTAR | Oil | 1.30 | 0.22 | 0.06 | 162.5 pixels | 506195 | | ||
*** pixel size for 2560 x 1920 images (Full Frame)** | *** pixel size for 2560 x 1920 images (Full Frame)** | ||
- | + | ==== Camera ==== | |
- | + | ^ Model ^ Type ^ Frame Size ^ Pixel Size (µm) ^ | |
- | **Upon request:** | + | | [[https://www.leica-microsystems.com/products/microscope-cameras/p/leica-dfc450-c/|Leica DFC450]] | color CCD | 2560 × 1920 | 3.4 x 3.4 | |
- | + | ||
- | ^ Magnification ^ Model ^ Type ^ NA ^ WD (mm) ^ pixel size (μm) ^ 10 μm = ^ | + | |
- | | 5x | N PLAN | Dry | 0.12 | 14 | 1.23 | 8.1 pixels | | + | |
- | | 63x | HCX PL APO | Oil | 1.32-0.60 | 0.1 | 0.1 | 102 pixels | | + | |
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[[resources|Back to the Resources page]] | [[resources|Back to the Resources page]] |