ivis_lumina
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ivis_lumina [2020/01/01 23:29] bioimaging |
ivis_lumina [2020/02/17 11:48] bioimaging [System overview] |
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| An adjustable field of view from 5 – 12.5 cm allows imaging of up to 3 mice or a medium size rat and also accommodates standard microtiter plates for //in vitro// imaging. The system includes premium animal handling features such as a heated sample shelf, gas anesthesia connections and manifold. | An adjustable field of view from 5 – 12.5 cm allows imaging of up to 3 mice or a medium size rat and also accommodates standard microtiter plates for //in vitro// imaging. The system includes premium animal handling features such as a heated sample shelf, gas anesthesia connections and manifold. | ||
| - | {{warning.jpg?20|}} Data files older than **3 months** will be automatically deleted on this system, please copy your data to the iMM server using the desktop link. | + | {{:warning.png?25|}} Data files older than **3 months** will be automatically deleted on this system, please copy your data to the iMM server using the desktop link. |
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| + | ===== System booking rules ===== | ||
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| + | {{:warning.png?25|}} You can book the system with **28 days** in advance. | ||
| ==== Filtersets ==== | ==== Filtersets ==== | ||
| - | ^ Filter Set ^ Label ^ Background Passband (nm) ^ Excitation Passband (nm) ^ Emission Passband (nm) ^ Dyes, Fluorescent Proteins, and Quantum Dots ^ | + | ^ Filter Set ^ Label ^ Background Passband (nm) ^ Excitation Passband (nm) ^ Emission Passband (nm) ^ Dyes / Fluorescent Proteins ^ |
| | 1 | Green | 410–440 | 445–490 | 515–575 | GFP, EGFP, FITC | | | 1 | Green | 410–440 | 445–490 | 515–575 | GFP, EGFP, FITC | | ||
| - | | 2 | Red | 460–490 | 500–550 | 575–650 | DsRed, PKH26, Qdot® 605 | | + | | 2 | Red | 460–490 | 500–550 | 575–650 | DsRed, PKH26 | |
| - | | 3 | Far-red | 580–610 | 615–665 | 695–770 | Cy5.5, Qdot® 705 | | + | | 3 | Far-red | 580–610 | 615–665 | 695–770 | Cy5.5 | |
| - | | 4 | NIR | 665–695 | 705–780 | 810–885 | ICG, Qdot® 800 | | + | | 4 | NIR | 665–695 | 705–780 | 810–885 | ICG | |
| ===== System Turn On Procedures ===== | ===== System Turn On Procedures ===== | ||
| - | * The system and computer should be turned on. If not please contact the [[imm-bioimaging@medicina.ulisboa.pt|Bioimaging Facility]]. | + | * The system and computer should be turned on. If not please contact the [[imm-bioimaging@medicina.ulisboa.pt|Bioimaging Unit]]. |
| * Check if the light source power supply is turned on (small box between the black box and the monitor). | * Check if the light source power supply is turned on (small box between the black box and the monitor). | ||
| * If the computer shows the login screen, use the username ''administrator'' and password ''password''. | * If the computer shows the login screen, use the username ''administrator'' and password ''password''. | ||
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| * Also clean with ethanol and paper tissues any other place (except the camera lenses or any optical components) that might have been in contact with tissues or fluids. | * Also clean with ethanol and paper tissues any other place (except the camera lenses or any optical components) that might have been in contact with tissues or fluids. | ||
| - | For a complete shutdown contact the [[imm-bioimaging@medicina.ulisboa.pt|Bioimaging Facility]]. | + | For a complete shutdown contact the [[imm-bioimaging@medicina.ulisboa.pt|Bioimaging Unit]]. |
| ===== Imaging Basics ===== | ===== Imaging Basics ===== | ||
ivis_lumina.txt · Last modified: 2023/03/27 13:09 by bioimaging
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