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ivis_lumina [2016/04/09 04:22] bioimaging [System overview] |
ivis_lumina [2020/10/31 01:48] bioimaging |
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- | <img src="/facility/bioimaging/lib/exe/fetch.php?media=ivis_lumina_name.jpg"> | + | <img src="/facility/bioimaging/lib/exe/fetch.php?media=ivis_lumina_name_new.png"> |
<table style="border:0px solid white;"> | <table style="border:0px solid white;"> | ||
<tr style="border:0px solid white;"> | <tr style="border:0px solid white;"> | ||
<td style="border:0px solid white;"> | <td style="border:0px solid white;"> | ||
- | <a href="/facility/bioimaging/lib/exe/fetch.php?media=ivis_lumina_wiki.jpg"><img src="/facility/bioimaging/lib/exe/fetch.php?media=ivis_lumina_wiki.jpg" width=300></a> | + | <a href="/facility/bioimaging/lib/exe/fetch.php?media=ivis_lumina_wiki_new.png"><img src="/facility/bioimaging/lib/exe/fetch.php?media=ivis_lumina_wiki_new.png" width=300></a> |
<td style="border:0px solid white;"> <p style="line-height:1.8"> | <td style="border:0px solid white;"> <p style="line-height:1.8"> | ||
- | <b>Location</b>: <a href="/facility/bioimaging/lib/exe/fetch.php?media=ivis_lumina_map.jpg">Room P2-A-3</a> (<img src="/facility/bioimaging/lib/exe/fetch.php?media=telephone_icon.gif" width=15> 47200) <br> | + | <b>Location</b>: <a href="/facility/bioimaging/lib/exe/fetch.php?media=ivis_lumina_map_new.png">Room P2-A-3</a> (<img src="/facility/bioimaging/lib/exe/fetch.php?media=phone_neg.png" width=15> 47200) <br> |
<b>Manufacturer</b>: <a href="http://www.perkinelmer.com/corporate/what-we-do/markets/life-sciences" target="_blank">Perkin Elmer</a> (Caliper LifeSciences)<br> | <b>Manufacturer</b>: <a href="http://www.perkinelmer.com/corporate/what-we-do/markets/life-sciences" target="_blank">Perkin Elmer</a> (Caliper LifeSciences)<br> | ||
<b>Model</b>: IVIS Lumina<br> | <b>Model</b>: IVIS Lumina<br> | ||
+ | <b>Nickname</b>: "Lumina"<br> | ||
<b>Software</b>: Living Image 3.0<br> | <b>Software</b>: Living Image 3.0<br> | ||
<b>Year</b>: 2007 <br> | <b>Year</b>: 2007 <br> | ||
- | <b>SN</b>: 150715N4196<br> | + | <b>SN</b>: IS0715N4196<br> |
  <br> |   <br> | ||
  <br> |   <br> | ||
  <br> |   <br> | ||
- |   <br> | + | → <a href="https://my.agendo.science/calendar/?id=MzVjYWxlbmRhcnM="><img src="/facility/bioimaging/lib/exe/fetch.php?media=date.png"> IVIS Lumina Booking</a> |
- | → <a href="https://imm.medicina.ulisboa.pt/intranet/booking/month.php?user=IVISLUMIN&rid=14"><img src="/facility/bioimaging/lib/exe/fetch.php?media=date.png"> IVIS Lumina Booking</a> | + | |
- | <br> | + | |
- | → <a href="/facility/bioimaging/doku.php?id=ivis_lumina_usage"><img src="/facility/bioimaging/lib/exe/fetch.php?media=chart_line.png"> IVIS Lumina Usage Statistics</a> | + | |
<br> | <br> | ||
- | → <a href="/facility/bioimaging/doku.php?id=ivis_lumina_repair"><img src="/facility/bioimaging/lib/exe/fetch.php?media=repair-icon.gif" width=16> IVIS Lumina Repair History</a> | + | → <a href="/facility/bioimaging/doku.php?id=ivis_lumina_quality"><img src="/facility/bioimaging/lib/exe/fetch.php?media=list-icon.png" width=18> IVIS Lumina Quality Control</a> |
- | </p> | + | <br> |
+ | → <a href="/facility/bioimaging/doku.php?id=ivis_lumina_usage"><img src="/facility/bioimaging/lib/exe/fetch.php?media=chart_line.png"> IVIS Lumina Usage Statistics</a> | ||
+ | </p> | ||
</td> | </td> | ||
</tr> | </tr> | ||
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An adjustable field of view from 5 – 12.5 cm allows imaging of up to 3 mice or a medium size rat and also accommodates standard microtiter plates for //in vitro// imaging. The system includes premium animal handling features such as a heated sample shelf, gas anesthesia connections and manifold. | An adjustable field of view from 5 – 12.5 cm allows imaging of up to 3 mice or a medium size rat and also accommodates standard microtiter plates for //in vitro// imaging. The system includes premium animal handling features such as a heated sample shelf, gas anesthesia connections and manifold. | ||
- | ^ Filter Set ^ Label ^ Background Passband (nm) ^ Excitation Passband (nm) ^ Emission Passband (nm) ^ Dyes, Fluorescent Proteins, and Quantum Dots ^ | + | {{:warning.png?25|}} Data files older than **3 months** will be automatically deleted on this system, please copy your data to the iMM server using the desktop link. |
+ | |||
+ | ===== System booking rules ===== | ||
+ | |||
+ | {{:warning.png?25|}} You can book the system with **28 days** in advance. | ||
+ | |||
+ | ==== Filtersets ==== | ||
+ | |||
+ | ^ Filter Set ^ Label ^ Background Passband (nm) ^ Excitation Passband (nm) ^ Emission Passband (nm) ^ Dyes / Fluorescent Proteins ^ | ||
| 1 | Green | 410–440 | 445–490 | 515–575 | GFP, EGFP, FITC | | | 1 | Green | 410–440 | 445–490 | 515–575 | GFP, EGFP, FITC | | ||
- | | 2 | Red | 460–490 | 500–550 | 575–650 | DsRed, PKH26, Qdot® 605 | | + | | 2 | Red | 460–490 | 500–550 | 575–650 | DsRed, PKH26 | |
- | | 3 | Far-red | 580–610 | 615–665 | 695–770 | Cy5.5, Qdot® 705 | | + | | 3 | Far-red | 580–610 | 615–665 | 695–770 | Cy5.5 | |
- | | 4 | NIR | 665–695 | 705–780 | 810–885 | ICG, Qdot® 800 | | + | | 4 | NIR | 665–695 | 705–780 | 810–885 | ICG | |
===== System Turn On Procedures ===== | ===== System Turn On Procedures ===== | ||
- | * The system and computer should be turned on. If not please contact the [[imm-bioimaging@medicina.ulisboa.pt|Bioimaging Facility]]. | + | * The system and computer should be turned on. If not please contact the [[imm-bioimaging@medicina.ulisboa.pt|Bioimaging Unit]]. |
* Check if the light source power supply is turned on (small box between the black box and the monitor). | * Check if the light source power supply is turned on (small box between the black box and the monitor). | ||
* If the computer shows the login screen, use the username ''administrator'' and password ''password''. | * If the computer shows the login screen, use the username ''administrator'' and password ''password''. | ||
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* Also clean with ethanol and paper tissues any other place (except the camera lenses or any optical components) that might have been in contact with tissues or fluids. | * Also clean with ethanol and paper tissues any other place (except the camera lenses or any optical components) that might have been in contact with tissues or fluids. | ||
- | For a complete shutdown contact the [[imm-bioimaging@medicina.ulisboa.pt|Bioimaging Facility]]. | + | For a complete shutdown contact the [[imm-bioimaging@medicina.ulisboa.pt|Bioimaging Unit]]. |
===== Imaging Basics ===== | ===== Imaging Basics ===== | ||
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* It is advisable to pre-screen all accessory items by imaging them alone, before imaging them with samples under study. | * It is advisable to pre-screen all accessory items by imaging them alone, before imaging them with samples under study. | ||
* Try not to use powdered gloves when working with the equipment. | * Try not to use powdered gloves when working with the equipment. | ||
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[[resources|Back to the Resources page]] | [[resources|Back to the Resources page]] |