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3i_marianas_sdc [2020/06/01 19:21] bioimaging |
3i_marianas_sdc [2020/10/27 18:23] bioimaging [Microscope Turn On Procedure] |
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<b>Model</b>: <a href="https://www.intelligent-imaging.com/marianas" target="_blank">Marianas</a><br> | <b>Model</b>: <a href="https://www.intelligent-imaging.com/marianas" target="_blank">Marianas</a><br> | ||
<b>Nickname</b>: "Marianas"<br> | <b>Nickname</b>: "Marianas"<br> | ||
- | <b>Software</b>: SlideBook 6.0.4<br> | + | <b>Software</b>: SlideBook 6.0.19<br> |
<b>Year</b>: 2012 <br> | <b>Year</b>: 2012 <br> | ||
<b>SN</b>: 1648 <br> | <b>SN</b>: 1648 <br> | ||
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^ Model ^ Frame Size ^ Pixel Size (µm) ^ Quantum Efficiency ^ | ^ Model ^ Frame Size ^ Pixel Size (µm) ^ Quantum Efficiency ^ | ||
| [[https://www.photometrics.com/wp-content/uploads/2019/10/Evolve512-Datasheet.pdf|Photometrics Evolve 512 EMCCD]] | 512 x 512 | 16 x 16 | > 90% | | | [[https://www.photometrics.com/wp-content/uploads/2019/10/Evolve512-Datasheet.pdf|Photometrics Evolve 512 EMCCD]] | 512 x 512 | 16 x 16 | > 90% | | ||
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===== Microscope Turn On Procedure ===== | ===== Microscope Turn On Procedure ===== | ||
* Turn on the fluorescence lamp (if needed) | * Turn on the fluorescence lamp (if needed) | ||
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* Turn on the secondary power supply switch (white socket outlet underneath the touchscreen) | * Turn on the secondary power supply switch (white socket outlet underneath the touchscreen) | ||
{{::marianas_power2.png|}} | {{::marianas_power2.png|}} | ||
- | * Turn on incubation and CO2 on the touchscreen (if needed) | + | * Turn on incubation and CO2 in the touchscreen (if needed) |
{{marianas_power6.jpg?|}} | {{marianas_power6.jpg?|}} | ||
* Turn on the lasers you need to use (using the key and the power switches) | * Turn on the lasers you need to use (using the key and the power switches) | ||
{{::marianas_power7.png?160|}} | {{::marianas_power7.png?160|}} | ||
* Turn on the computer | * Turn on the computer | ||
+ | * Login in to Windows (Bioimaging User) | ||
* Wait 2 min to allow for system components detection | * Wait 2 min to allow for system components detection | ||
* Start the **SlideBook** software | * Start the **SlideBook** software | ||
- | **Warning**: If you need to change the stage adaptor, please contact the <html><a href="/facility/bioimaging/doku.php?id=about">Bioimaging Unit</a> (<b>imm-bioimaging@medicina.ulisboa.pt</b> | <img src="/facility/bioimaging/lib/exe/fetch.php?media=phone_neg.png" width=15> 47222</html>) | + | **Warning**: If you need to change the stage adapter, please contact the <html><a href="/facility/bioimaging/doku.php?id=about">Bioimaging Unit</a> (<b>imm-bioimaging@medicina.ulisboa.pt</b> | <img src="/facility/bioimaging/lib/exe/fetch.php?media=phone_neg.png" width=15> 47222</html>) |
===== Microscope Turn Off Procedure ===== | ===== Microscope Turn Off Procedure ===== | ||
- | * Remove your sample from the stage/piezo holder | + | If there is another user for this microscope in the __next hour__: |
+ | * Exit the SlideBook software, leave the lasers on | ||
+ | * Log off the computer | ||
+ | * Clean up immersion objectives | ||
+ | Else: | ||
* Exit the **SlideBook** software | * Exit the **SlideBook** software | ||
- | * Switch off the computer | + | * Shut down the computer |
* Turn off incubation in the touchscreen (if used) | * Turn off incubation in the touchscreen (if used) | ||
* Turn off CO2 (if used) | * Turn off CO2 (if used) |