3i_marianas_sdc
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| Both sides previous revision Previous revision Next revision | Previous revision Next revision Both sides next revision | ||
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3i_marianas_sdc [2020/03/01 15:58] bioimaging |
3i_marianas_sdc [2020/10/27 18:01] bioimaging |
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| <b>Model</b>: <a href="https://www.intelligent-imaging.com/marianas" target="_blank">Marianas</a><br> | <b>Model</b>: <a href="https://www.intelligent-imaging.com/marianas" target="_blank">Marianas</a><br> | ||
| <b>Nickname</b>: "Marianas"<br> | <b>Nickname</b>: "Marianas"<br> | ||
| - | <b>Software</b>: SlideBook 6.0.4<br> | + | <b>Software</b>: SlideBook 6.0.19<br> |
| <b>Year</b>: 2012 <br> | <b>Year</b>: 2012 <br> | ||
| <b>SN</b>: 1648 <br> | <b>SN</b>: 1648 <br> | ||
| Line 16: | Line 16: | ||
|   <br> |   <br> | ||
|   <br> |   <br> | ||
| - | → <a href="https://imm.medicina.ulisboa.pt/intranet/booking/111"><img src="/facility/bioimaging/lib/exe/fetch.php?media=date.png"> 3i Marianas SDC Booking</a> | + | → <a href="https://my.agendo.science/calendar/?id=MjdjYWxlbmRhcnM="><img src="/facility/bioimaging/lib/exe/fetch.php?media=date.png"> 3i Marianas SDC Booking</a> |
| <br> | <br> | ||
| → <a href="/facility/bioimaging/doku.php?id=3i_marianas_sdc_quality"><img src="/facility/bioimaging/lib/exe/fetch.php?media=list-icon.png" width=18> 3i Marianas SDC Quality Control</a> | → <a href="/facility/bioimaging/doku.php?id=3i_marianas_sdc_quality"><img src="/facility/bioimaging/lib/exe/fetch.php?media=list-icon.png" width=18> 3i Marianas SDC Quality Control</a> | ||
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| ^ Model ^ Frame Size ^ Pixel Size (µm) ^ Quantum Efficiency ^ | ^ Model ^ Frame Size ^ Pixel Size (µm) ^ Quantum Efficiency ^ | ||
| | [[https://www.photometrics.com/wp-content/uploads/2019/10/Evolve512-Datasheet.pdf|Photometrics Evolve 512 EMCCD]] | 512 x 512 | 16 x 16 | > 90% | | | [[https://www.photometrics.com/wp-content/uploads/2019/10/Evolve512-Datasheet.pdf|Photometrics Evolve 512 EMCCD]] | 512 x 512 | 16 x 16 | > 90% | | ||
| + | |||
| ===== Microscope Turn On Procedure ===== | ===== Microscope Turn On Procedure ===== | ||
| * Turn on the fluorescence lamp (if needed) | * Turn on the fluorescence lamp (if needed) | ||
| Line 82: | Line 83: | ||
| * Turn on the secondary power supply switch (white socket outlet underneath the touchscreen) | * Turn on the secondary power supply switch (white socket outlet underneath the touchscreen) | ||
| {{::marianas_power2.png|}} | {{::marianas_power2.png|}} | ||
| - | * Turn on incubation and CO2 on the touchscreen (if needed) | + | * Turn on incubation and CO2 in the touchscreen (if needed) |
| {{marianas_power6.jpg?|}} | {{marianas_power6.jpg?|}} | ||
| * Turn on the lasers you need to use (using the key and the power switches) | * Turn on the lasers you need to use (using the key and the power switches) | ||
| Line 93: | Line 94: | ||
| ===== Microscope Turn Off Procedure ===== | ===== Microscope Turn Off Procedure ===== | ||
| - | * Remove your sample from the stage/piezo holder | + | If there is another user for this microscope in the __next hour__: |
| + | * Exit the SlideBook software, leave the lasers on | ||
| + | * Log off the computer | ||
| + | * Clean up immersion objectives | ||
| + | Else: | ||
| * Exit the **SlideBook** software | * Exit the **SlideBook** software | ||
| - | * Switch off the computer | + | * Shut down the computer |
| * Turn off incubation in the touchscreen (if used) | * Turn off incubation in the touchscreen (if used) | ||
| * Turn off CO2 (if used) | * Turn off CO2 (if used) | ||
3i_marianas_sdc.txt · Last modified: 2024/04/01 23:50 by bioimaging
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