3i_marianas_sdc
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3i_marianas_sdc [2020/01/01 17:31] bioimaging |
3i_marianas_sdc [2020/02/26 11:09] bioimaging [Objectives] |
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| ==== Objectives ==== | ==== Objectives ==== | ||
| ^ Magnification ^ Model ^ Immersion ^ NA ^ WD (mm) ^ Reference ^ | ^ Magnification ^ Model ^ Immersion ^ NA ^ WD (mm) ^ Reference ^ | ||
| - | | 20x | Plan-Apochromat | Air | 0.8 | 0.55 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420650-9902-000/Objective-Plan-Apochromat-20x-0.8-M27#|420650-9902-000]] | | + | | 10x | EC Plan-Neofluar | Air | 0.30 | 5.5 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420340-9900-000/Objective-EC-Plan-Neofluar-10x-0.30-M27|420340-9900-000]] | |
| + | | 20x | Plan-Apochromat | Air | 0.8 | 0.55 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420650-9901-000/Objective-Plan-Apochromat-20x-0.8-M27|420650-9901-000]] | | ||
| + | | 40x | LD C-Apochromat Corr | Water | 1.1 | 0.62 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/421867-9970-000/Objective-LD-C-Apochromat-40x-1.1-W-Corr-M27|421867-9970-000]] | | ||
| | 63x | Plan-Apochromat | Oil | 1.40 | 0.19 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420780-9900-000/Objective-Plan-Apochromat-63x-1.4-Oil-M27|420780-9900-000]] | | | 63x | Plan-Apochromat | Oil | 1.40 | 0.19 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420780-9900-000/Objective-Plan-Apochromat-63x-1.4-Oil-M27|420780-9900-000]] | | ||
| | 100x | Plan-Apochromat | Oil | 1.40 | 0.17 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420790-9901-000/Objective-Plan-Apochromat-100x-1.40-Oil-M27|420790-9901-000]] | | | 100x | Plan-Apochromat | Oil | 1.40 | 0.17 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420790-9901-000/Objective-Plan-Apochromat-100x-1.40-Oil-M27|420790-9901-000]] | | ||
| Line 54: | Line 56: | ||
| **Upon request:** | **Upon request:** | ||
| ^ Magnification ^ Model ^ Immersion ^ NA ^ WD (mm) ^ Reference ^ | ^ Magnification ^ Model ^ Immersion ^ NA ^ WD (mm) ^ Reference ^ | ||
| - | | 25x | LCI Plan-NeoFluar Imm Corr DIC | Oil/Glyc/W | 0.8 | 0.21 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420852-9973-000/Objective-LCI-Plan-Neofluar-25x-0.8-Imm-Corr-DIC-M27|420852-9973-000]] | | + | | 25x | LCI Plan-Neofluar Corr DIC | Oil/Glyc/W | 0.80 | 0.21 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420852-9972-000/Objective-LCI-Plan-Neofluar-25x-0.8-Imm-Corr-DIC-M27|420852-9972-000]] | |
| | 40x | Plan-Apochromat Corr | Air | 0.95 | 0.25 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420660-9970-000/Objective-Plan-Apochromat-40x-0.95-Corr-M27|420660-9970-000]] | | | 40x | Plan-Apochromat Corr | Air | 0.95 | 0.25 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420660-9970-000/Objective-Plan-Apochromat-40x-0.95-Corr-M27|420660-9970-000]] | | ||
| - | ==== Emission Filtersets ==== | + | ==== Emission Filters ==== |
| ^ Setting ^ Transmission ^ | ^ Setting ^ Transmission ^ | ||
| | 1: 445 | 422-477 nm | | | 1: 445 | 422-477 nm | | ||
| Line 74: | Line 76: | ||
| | [[https://www.photometrics.com/wp-content/uploads/2019/10/Evolve512-Datasheet.pdf|Photometrics Evolve 512 EMCCD]] | 512 x 512 | 16 x 16 | > 90% | | | [[https://www.photometrics.com/wp-content/uploads/2019/10/Evolve512-Datasheet.pdf|Photometrics Evolve 512 EMCCD]] | 512 x 512 | 16 x 16 | > 90% | | ||
| ===== Microscope Turn On Procedure ===== | ===== Microscope Turn On Procedure ===== | ||
| - | * Check that the main power supply switch is on (should be on by default) | ||
| - | {{marianas_power1.jpg?|}} | ||
| * Turn on the fluorescence lamp (if needed) | * Turn on the fluorescence lamp (if needed) | ||
| {{marianas_power5.jpg?|}} | {{marianas_power5.jpg?|}} | ||
| - | * Turn on the power switch distributor (green) and the component power switches (red) **one at a time** | + | * Turn on the power switch distributor (green) and the component power switches (red) **one at a time** from left to right |
| {{marianas_power3.jpg?|}} | {{marianas_power3.jpg?|}} | ||
| - | * Turn on the secondary power supply switch | + | * Turn on the secondary power supply switch (white socket outlet underneath the touchscreen) |
| - | {{marianas_power2.jpg?|}} | + | |
| - | * Turn on the Yokogawa spinning disk scanner (using the key) | + | |
| - | {{marianas_power4.jpg?|}} | + | |
| * Turn on incubation and CO2 on the touchscreen (if needed) | * Turn on incubation and CO2 on the touchscreen (if needed) | ||
| {{marianas_power6.jpg?|}} | {{marianas_power6.jpg?|}} | ||
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| * Exit the **SlideBook** software | * Exit the **SlideBook** software | ||
| * Switch off the computer | * Switch off the computer | ||
| - | * Turn off all system components (follow the Turn On Procedure in reverse) | + | * Turn off incubation in the touchscreen (if used) |
| + | * Turn off CO2 (if used) | ||
| + | * Switch all lasers off (first on the switch and finally by turning the key) | ||
| + | * Turn off the fluorescent lamp | ||
| + | * Turn off the secondary power supply switch (white socket outlet underneath the touchscreen) | ||
| + | * Turn off the component power switches (red) **one at a time** from right to left and the power switch distributor (green) | ||
| ---- | ---- | ||
| [[resources|Back to the Resources page]] | [[resources|Back to the Resources page]] | ||
3i_marianas_sdc.txt · Last modified: 2024/04/01 23:50 by bioimaging
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