3i_marianas_sdc
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| Both sides previous revision Previous revision Next revision | Previous revision Next revision Both sides next revision | ||
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3i_marianas_sdc [2020/01/01 17:04] bioimaging [System overview] |
3i_marianas_sdc [2020/01/21 18:26] bioimaging [Microscope Turn On Procedure] |
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| **Upon request:** | **Upon request:** | ||
| ^ Magnification ^ Model ^ Immersion ^ NA ^ WD (mm) ^ Reference ^ | ^ Magnification ^ Model ^ Immersion ^ NA ^ WD (mm) ^ Reference ^ | ||
| - | | 25x | LCI Plan-NeoFluar | Oil/Glyc/W | 0.8 | 0.21 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420852-9973-000/Objective-LCI-Plan-Neofluar-25x-0.8-Imm-Corr-DIC-M27|420852-9973-000]] | | + | | 25x | LCI Plan-Neofluar Corr DIC | Oil/Glyc/W | 0.80 | 0.21 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420852-9972-000/Objective-LCI-Plan-Neofluar-25x-0.8-Imm-Corr-DIC-M27|420852-9972-000]] | |
| - | | 40x | Plan-Apochromat | Air | 0.95 | 0.25 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420660-9970-000/Objective-Plan-Apochromat-40x-0.95-Corr-M27|420660-9970-000]] | | + | | 40x | Plan-Apochromat Corr | Air | 0.95 | 0.25 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420660-9970-000/Objective-Plan-Apochromat-40x-0.95-Corr-M27|420660-9970-000]] | |
| - | ==== Emission Filtersets ==== | + | ==== Emission Filters ==== |
| ^ Setting ^ Transmission ^ | ^ Setting ^ Transmission ^ | ||
| | 1: 445 | 422-477 nm | | | 1: 445 | 422-477 nm | | ||
| Line 72: | Line 72: | ||
| ==== Camera ==== | ==== Camera ==== | ||
| ^ Model ^ Frame Size ^ Pixel Size (µm) ^ Quantum Efficiency ^ | ^ Model ^ Frame Size ^ Pixel Size (µm) ^ Quantum Efficiency ^ | ||
| - | | Photometrics Evolve 512 EMCCD | 512 x 512 | 16 x 16 | > 90% | | + | | [[https://www.photometrics.com/wp-content/uploads/2019/10/Evolve512-Datasheet.pdf|Photometrics Evolve 512 EMCCD]] | 512 x 512 | 16 x 16 | > 90% | |
| ===== Microscope Turn On Procedure ===== | ===== Microscope Turn On Procedure ===== | ||
| * Check that the main power supply switch is on (should be on by default) | * Check that the main power supply switch is on (should be on by default) | ||
| Line 78: | Line 78: | ||
| * Turn on the fluorescence lamp (if needed) | * Turn on the fluorescence lamp (if needed) | ||
| {{marianas_power5.jpg?|}} | {{marianas_power5.jpg?|}} | ||
| - | * Turn on the power switch distributor (green) and the component power switches (red) **one at a time** | + | * Turn on the power switch distributor (green) and the component power switches (red) **one at a time** from left to right |
| {{marianas_power3.jpg?|}} | {{marianas_power3.jpg?|}} | ||
| - | * Turn on the secondary power supply switch | + | * Turn on the secondary power supply switch (white socket outlet underneath the touchscreen) |
| - | {{marianas_power2.jpg?|}} | + | |
| * Turn on the Yokogawa spinning disk scanner (using the key) | * Turn on the Yokogawa spinning disk scanner (using the key) | ||
| {{marianas_power4.jpg?|}} | {{marianas_power4.jpg?|}} | ||
| Line 98: | Line 97: | ||
| * Exit the **SlideBook** software | * Exit the **SlideBook** software | ||
| * Switch off the computer | * Switch off the computer | ||
| + | * Turn off incubation in the touchscreen (if used) | ||
| + | * Turn off CO2 (if used) | ||
| + | * Switch all lasers off (first on the switch and finally by turning the key) | ||
| + | * Turn off the fluorescent lamp | ||
| + | * Turn off the secondary power supply switch (white socket outlet underneath the touchscreen) | ||
| + | * | ||
| * Turn off all system components (follow the Turn On Procedure in reverse) | * Turn off all system components (follow the Turn On Procedure in reverse) | ||
3i_marianas_sdc.txt · Last modified: 2024/04/01 23:50 by bioimaging
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