3i_marianas_sdc
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| Both sides previous revision Previous revision Next revision | Previous revision Next revision Both sides next revision | ||
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3i_marianas_sdc [2020/01/01 17:04] bioimaging [System overview] |
3i_marianas_sdc [2020/01/17 13:07] bioimaging [Emission Filtersets] |
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| **Upon request:** | **Upon request:** | ||
| ^ Magnification ^ Model ^ Immersion ^ NA ^ WD (mm) ^ Reference ^ | ^ Magnification ^ Model ^ Immersion ^ NA ^ WD (mm) ^ Reference ^ | ||
| - | | 25x | LCI Plan-NeoFluar | Oil/Glyc/W | 0.8 | 0.21 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420852-9973-000/Objective-LCI-Plan-Neofluar-25x-0.8-Imm-Corr-DIC-M27|420852-9973-000]] | | + | | 25x | LCI Plan-Neofluar Corr DIC | Oil/Glyc/W | 0.80 | 0.21 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420852-9972-000/Objective-LCI-Plan-Neofluar-25x-0.8-Imm-Corr-DIC-M27|420852-9972-000]] | |
| - | | 40x | Plan-Apochromat | Air | 0.95 | 0.25 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420660-9970-000/Objective-Plan-Apochromat-40x-0.95-Corr-M27|420660-9970-000]] | | + | | 40x | Plan-Apochromat Corr | Air | 0.95 | 0.25 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420660-9970-000/Objective-Plan-Apochromat-40x-0.95-Corr-M27|420660-9970-000]] | |
| - | ==== Emission Filtersets ==== | + | ==== Emission Filters ==== |
| ^ Setting ^ Transmission ^ | ^ Setting ^ Transmission ^ | ||
| | 1: 445 | 422-477 nm | | | 1: 445 | 422-477 nm | | ||
| Line 72: | Line 72: | ||
| ==== Camera ==== | ==== Camera ==== | ||
| ^ Model ^ Frame Size ^ Pixel Size (µm) ^ Quantum Efficiency ^ | ^ Model ^ Frame Size ^ Pixel Size (µm) ^ Quantum Efficiency ^ | ||
| - | | Photometrics Evolve 512 EMCCD | 512 x 512 | 16 x 16 | > 90% | | + | | [[https://www.photometrics.com/wp-content/uploads/2019/10/Evolve512-Datasheet.pdf|Photometrics Evolve 512 EMCCD]] | 512 x 512 | 16 x 16 | > 90% | |
| ===== Microscope Turn On Procedure ===== | ===== Microscope Turn On Procedure ===== | ||
| * Check that the main power supply switch is on (should be on by default) | * Check that the main power supply switch is on (should be on by default) | ||
3i_marianas_sdc.txt · Last modified: 2024/04/01 23:50 by bioimaging
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