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3i_marianas_sdc [2020/01/01 16:55] bioimaging [Objectives] |
3i_marianas_sdc [2020/01/21 18:26] bioimaging [Microscope Turn On Procedure] |
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* **Microscope**: [[http://www.zeiss.com/microscopy/en_de/products/light-microscopes/axio-observer-for-biology.html|Zeiss Axio Observer]] | * **Microscope**: [[http://www.zeiss.com/microscopy/en_de/products/light-microscopes/axio-observer-for-biology.html|Zeiss Axio Observer]] | ||
* **Confocal scanner**: [[https://www.yokogawa.com/eu/solutions/products-platforms/life-science/spinning-disk-confocal/csu-x1-confocal-scanner-unit/|Yokogawa CSU-X1]] | * **Confocal scanner**: [[https://www.yokogawa.com/eu/solutions/products-platforms/life-science/spinning-disk-confocal/csu-x1-confocal-scanner-unit/|Yokogawa CSU-X1]] | ||
- | * **Camera**: [[https://www.photometrics.com/wp-content/uploads/2019/10/Evolve512-Datasheet.pdf|Evolve 512 EMCCD]] | + | * **Camera**: [[https://www.photometrics.com/wp-content/uploads/2019/10/Evolve512-Datasheet.pdf|Photometrics Evolve 512 EMCCD]] |
{{:warning.png?25|}} Data files older than **1 month** will be automatically deleted on this system, please copy your data to the iMM server using the desktop link. | {{:warning.png?25|}} Data files older than **1 month** will be automatically deleted on this system, please copy your data to the iMM server using the desktop link. | ||
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==== Objectives ==== | ==== Objectives ==== | ||
^ Magnification ^ Model ^ Immersion ^ NA ^ WD (mm) ^ Reference ^ | ^ Magnification ^ Model ^ Immersion ^ NA ^ WD (mm) ^ Reference ^ | ||
- | | 20x | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420650-9902-000/Objective-Plan-Apochromat-20x-0.8-M27#|Plan-Apochromat]] | Air | 0.8 | 0.55 | 420650-9902-000 | | + | | 20x | Plan-Apochromat | Air | 0.8 | 0.55 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420650-9902-000/Objective-Plan-Apochromat-20x-0.8-M27#|420650-9902-000]] | |
- | | 63x | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420780-9900-000/Objective-Plan-Apochromat-63x-1.4-Oil-M27|Plan-Apochromat]] | Oil | 1.40 | 0.19 | 420780-9900-000 | | + | | 63x | Plan-Apochromat | Oil | 1.40 | 0.19 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420780-9900-000/Objective-Plan-Apochromat-63x-1.4-Oil-M27|420780-9900-000]] | |
- | | 100x | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420790-9901-000/Objective-Plan-Apochromat-100x-1.40-Oil-M27|Plan-Apochromat]] | Oil | 1.40 | 0.17 | 420790-9901-000 | | + | | 100x | Plan-Apochromat | Oil | 1.40 | 0.17 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420790-9901-000/Objective-Plan-Apochromat-100x-1.40-Oil-M27|420790-9901-000]] | |
**Upon request:** | **Upon request:** | ||
^ Magnification ^ Model ^ Immersion ^ NA ^ WD (mm) ^ Reference ^ | ^ Magnification ^ Model ^ Immersion ^ NA ^ WD (mm) ^ Reference ^ | ||
- | | 25x | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420852-9973-000/Objective-LCI-Plan-Neofluar-25x-0.8-Imm-Corr-DIC-M27|LCI Plan-NeoFluar]] | Oil/Glyc/W | 0.8 | 0.21 | 420852-9973-000 | | + | | 25x | LCI Plan-Neofluar Corr DIC | Oil/Glyc/W | 0.80 | 0.21 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420852-9972-000/Objective-LCI-Plan-Neofluar-25x-0.8-Imm-Corr-DIC-M27|420852-9972-000]] | |
- | | 40x | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420660-9970-000/Objective-Plan-Apochromat-40x-0.95-Corr-M27|Plan-Apochromat]] | Air | 0.95 | 0.25 | 420660-9970-000 ^ | + | | 40x | Plan-Apochromat Corr | Air | 0.95 | 0.25 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420660-9970-000/Objective-Plan-Apochromat-40x-0.95-Corr-M27|420660-9970-000]] | |
- | ==== Emission Filtersets ==== | + | |
+ | ==== Emission Filters ==== | ||
^ Setting ^ Transmission ^ | ^ Setting ^ Transmission ^ | ||
| 1: 445 | 422-477 nm | | | 1: 445 | 422-477 nm | | ||
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| 10: 445 | 422-477 nm | | | 10: 445 | 422-477 nm | | ||
+ | ==== Camera ==== | ||
+ | ^ Model ^ Frame Size ^ Pixel Size (µm) ^ Quantum Efficiency ^ | ||
+ | | [[https://www.photometrics.com/wp-content/uploads/2019/10/Evolve512-Datasheet.pdf|Photometrics Evolve 512 EMCCD]] | 512 x 512 | 16 x 16 | > 90% | | ||
===== Microscope Turn On Procedure ===== | ===== Microscope Turn On Procedure ===== | ||
* Check that the main power supply switch is on (should be on by default) | * Check that the main power supply switch is on (should be on by default) | ||
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* Turn on the fluorescence lamp (if needed) | * Turn on the fluorescence lamp (if needed) | ||
{{marianas_power5.jpg?|}} | {{marianas_power5.jpg?|}} | ||
- | * Turn on the power switch distributor (green) and the component power switches (red) **one at a time** | + | * Turn on the power switch distributor (green) and the component power switches (red) **one at a time** from left to right |
{{marianas_power3.jpg?|}} | {{marianas_power3.jpg?|}} | ||
- | * Turn on the secondary power supply switch | + | * Turn on the secondary power supply switch (white socket outlet underneath the touchscreen) |
- | {{marianas_power2.jpg?|}} | + | |
* Turn on the Yokogawa spinning disk scanner (using the key) | * Turn on the Yokogawa spinning disk scanner (using the key) | ||
{{marianas_power4.jpg?|}} | {{marianas_power4.jpg?|}} | ||
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* Exit the **SlideBook** software | * Exit the **SlideBook** software | ||
* Switch off the computer | * Switch off the computer | ||
+ | * Turn off incubation in the touchscreen (if used) | ||
+ | * Turn off CO2 (if used) | ||
+ | * Switch all lasers off (first on the switch and finally by turning the key) | ||
+ | * Turn off the fluorescent lamp | ||
+ | * Turn off the secondary power supply switch (white socket outlet underneath the touchscreen) | ||
+ | * | ||
* Turn off all system components (follow the Turn On Procedure in reverse) | * Turn off all system components (follow the Turn On Procedure in reverse) | ||