3i_marianas_sdc
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| Both sides previous revision Previous revision Next revision | Previous revision Next revision Both sides next revision | ||
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3i_marianas_sdc [2020/01/01 16:41] bioimaging |
3i_marianas_sdc [2020/01/21 18:27] bioimaging [Microscope Turn Off Procedure] |
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| * **Microscope**: [[http://www.zeiss.com/microscopy/en_de/products/light-microscopes/axio-observer-for-biology.html|Zeiss Axio Observer]] | * **Microscope**: [[http://www.zeiss.com/microscopy/en_de/products/light-microscopes/axio-observer-for-biology.html|Zeiss Axio Observer]] | ||
| * **Confocal scanner**: [[https://www.yokogawa.com/eu/solutions/products-platforms/life-science/spinning-disk-confocal/csu-x1-confocal-scanner-unit/|Yokogawa CSU-X1]] | * **Confocal scanner**: [[https://www.yokogawa.com/eu/solutions/products-platforms/life-science/spinning-disk-confocal/csu-x1-confocal-scanner-unit/|Yokogawa CSU-X1]] | ||
| - | * **Camera**: [[https://www.photometrics.com/wp-content/uploads/2019/10/Evolve512-Datasheet.pdf|Evolve 512 EMCCD]] | + | * **Camera**: [[https://www.photometrics.com/wp-content/uploads/2019/10/Evolve512-Datasheet.pdf|Photometrics Evolve 512 EMCCD]] |
| {{:warning.png?25|}} Data files older than **1 month** will be automatically deleted on this system, please copy your data to the iMM server using the desktop link. | {{:warning.png?25|}} Data files older than **1 month** will be automatically deleted on this system, please copy your data to the iMM server using the desktop link. | ||
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| ==== Objectives ==== | ==== Objectives ==== | ||
| - | ^ Magnification ^ Model ^ Type ^ NA ^ WD (mm) ^ Reference ^ | + | ^ Magnification ^ Model ^ Immersion ^ NA ^ WD (mm) ^ Reference ^ |
| - | | 20x | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420650-9902-000/Objective-Plan-Apochromat-20x-0.8-M27#|Plan-Apochromat]] | Dry | 0.8 | 0.55 | 420650-9902-000 | | + | | 20x | Plan-Apochromat | Air | 0.8 | 0.55 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420650-9902-000/Objective-Plan-Apochromat-20x-0.8-M27#|420650-9902-000]] | |
| - | | 63x | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420780-9900-000/Objective-Plan-Apochromat-63x-1.4-Oil-M27|Plan-Apochromat]] | Oil | 1.40 | 0.19 | 420780-9900-000 | | + | | 63x | Plan-Apochromat | Oil | 1.40 | 0.19 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420780-9900-000/Objective-Plan-Apochromat-63x-1.4-Oil-M27|420780-9900-000]] | |
| - | | 100x | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420790-9901-000/Objective-Plan-Apochromat-100x-1.40-Oil-M27|Plan-Apochromat]] | Oil | 1.40 | 0.17 | 420790-9901-000 | | + | | 100x | Plan-Apochromat | Oil | 1.40 | 0.17 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420790-9901-000/Objective-Plan-Apochromat-100x-1.40-Oil-M27|420790-9901-000]] | |
| **Upon request:** | **Upon request:** | ||
| - | ^ Magnification ^ Model ^ Type ^ NA ^ WD (mm) ^ Reference ^ | + | ^ Magnification ^ Model ^ Immersion ^ NA ^ WD (mm) ^ Reference ^ |
| - | | 25x | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420852-9973-000/Objective-LCI-Plan-Neofluar-25x-0.8-Imm-Corr-DIC-M27|LCI Plan-NeoFluar]] | Oil/Glyc/W | 0.8 | 0.21 | 420852-9973-000 | | + | | 25x | LCI Plan-Neofluar Corr DIC | Oil/Glyc/W | 0.80 | 0.21 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420852-9972-000/Objective-LCI-Plan-Neofluar-25x-0.8-Imm-Corr-DIC-M27|420852-9972-000]] | |
| - | | 40x | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420660-9970-000/Objective-Plan-Apochromat-40x-0.95-Corr-M27|Plan-Apochromat]] | Dry | 0.95 | 0.25 | 420660-9970-000 ^ | + | | 40x | Plan-Apochromat Corr | Air | 0.95 | 0.25 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420660-9970-000/Objective-Plan-Apochromat-40x-0.95-Corr-M27|420660-9970-000]] | |
| - | ==== Emission Filtersets ==== | + | |
| + | ==== Emission Filters ==== | ||
| ^ Setting ^ Transmission ^ | ^ Setting ^ Transmission ^ | ||
| | 1: 445 | 422-477 nm | | | 1: 445 | 422-477 nm | | ||
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| | 10: 445 | 422-477 nm | | | 10: 445 | 422-477 nm | | ||
| + | ==== Camera ==== | ||
| + | ^ Model ^ Frame Size ^ Pixel Size (µm) ^ Quantum Efficiency ^ | ||
| + | | [[https://www.photometrics.com/wp-content/uploads/2019/10/Evolve512-Datasheet.pdf|Photometrics Evolve 512 EMCCD]] | 512 x 512 | 16 x 16 | > 90% | | ||
| ===== Microscope Turn On Procedure ===== | ===== Microscope Turn On Procedure ===== | ||
| * Check that the main power supply switch is on (should be on by default) | * Check that the main power supply switch is on (should be on by default) | ||
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| * Turn on the fluorescence lamp (if needed) | * Turn on the fluorescence lamp (if needed) | ||
| {{marianas_power5.jpg?|}} | {{marianas_power5.jpg?|}} | ||
| - | * Turn on the power switch distributor (green) and the component power switches (red) **one at a time** | + | * Turn on the power switch distributor (green) and the component power switches (red) **one at a time** from left to right |
| {{marianas_power3.jpg?|}} | {{marianas_power3.jpg?|}} | ||
| - | * Turn on the secondary power supply switch | + | * Turn on the secondary power supply switch (white socket outlet underneath the touchscreen) |
| - | {{marianas_power2.jpg?|}} | + | |
| * Turn on the Yokogawa spinning disk scanner (using the key) | * Turn on the Yokogawa spinning disk scanner (using the key) | ||
| {{marianas_power4.jpg?|}} | {{marianas_power4.jpg?|}} | ||
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| * Exit the **SlideBook** software | * Exit the **SlideBook** software | ||
| * Switch off the computer | * Switch off the computer | ||
| - | * Turn off all system components (follow the Turn On Procedure in reverse) | + | * Turn off incubation in the touchscreen (if used) |
| + | * Turn off CO2 (if used) | ||
| + | * Switch all lasers off (first on the switch and finally by turning the key) | ||
| + | * Turn off the fluorescent lamp | ||
| + | * Turn off the secondary power supply switch (white socket outlet underneath the touchscreen) | ||
| + | * Turn off the component power switches (red) **one at a time** from right to left and the power switch distributor (green) | ||
| ---- | ---- | ||
| [[resources|Back to the Resources page]] | [[resources|Back to the Resources page]] | ||
3i_marianas_sdc.txt · Last modified: 2024/04/01 23:50 by bioimaging
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