3i_marianas_sdc
Differences
This shows you the differences between two versions of the page.
| Both sides previous revision Previous revision Next revision | Previous revision Next revision Both sides next revision | ||
|
3i_marianas_sdc [2020/01/01 16:41] bioimaging |
3i_marianas_sdc [2020/01/01 17:21] bioimaging [Objectives] |
||
|---|---|---|---|
| Line 34: | Line 34: | ||
| * **Microscope**: [[http://www.zeiss.com/microscopy/en_de/products/light-microscopes/axio-observer-for-biology.html|Zeiss Axio Observer]] | * **Microscope**: [[http://www.zeiss.com/microscopy/en_de/products/light-microscopes/axio-observer-for-biology.html|Zeiss Axio Observer]] | ||
| * **Confocal scanner**: [[https://www.yokogawa.com/eu/solutions/products-platforms/life-science/spinning-disk-confocal/csu-x1-confocal-scanner-unit/|Yokogawa CSU-X1]] | * **Confocal scanner**: [[https://www.yokogawa.com/eu/solutions/products-platforms/life-science/spinning-disk-confocal/csu-x1-confocal-scanner-unit/|Yokogawa CSU-X1]] | ||
| - | * **Camera**: [[https://www.photometrics.com/wp-content/uploads/2019/10/Evolve512-Datasheet.pdf|Evolve 512 EMCCD]] | + | * **Camera**: [[https://www.photometrics.com/wp-content/uploads/2019/10/Evolve512-Datasheet.pdf|Photometrics Evolve 512 EMCCD]] |
| {{:warning.png?25|}} Data files older than **1 month** will be automatically deleted on this system, please copy your data to the iMM server using the desktop link. | {{:warning.png?25|}} Data files older than **1 month** will be automatically deleted on this system, please copy your data to the iMM server using the desktop link. | ||
| Line 47: | Line 47: | ||
| ==== Objectives ==== | ==== Objectives ==== | ||
| - | ^ Magnification ^ Model ^ Type ^ NA ^ WD (mm) ^ Reference ^ | + | ^ Magnification ^ Model ^ Immersion ^ NA ^ WD (mm) ^ Reference ^ |
| - | | 20x | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420650-9902-000/Objective-Plan-Apochromat-20x-0.8-M27#|Plan-Apochromat]] | Dry | 0.8 | 0.55 | 420650-9902-000 | | + | | 20x | Plan-Apochromat | Air | 0.8 | 0.55 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420650-9902-000/Objective-Plan-Apochromat-20x-0.8-M27#|420650-9902-000]] | |
| - | | 63x | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420780-9900-000/Objective-Plan-Apochromat-63x-1.4-Oil-M27|Plan-Apochromat]] | Oil | 1.40 | 0.19 | 420780-9900-000 | | + | | 63x | Plan-Apochromat | Oil | 1.40 | 0.19 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420780-9900-000/Objective-Plan-Apochromat-63x-1.4-Oil-M27|420780-9900-000]] | |
| - | | 100x | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420790-9901-000/Objective-Plan-Apochromat-100x-1.40-Oil-M27|Plan-Apochromat]] | Oil | 1.40 | 0.17 | 420790-9901-000 | | + | | 100x | Plan-Apochromat | Oil | 1.40 | 0.17 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420790-9901-000/Objective-Plan-Apochromat-100x-1.40-Oil-M27|420790-9901-000]] | |
| **Upon request:** | **Upon request:** | ||
| - | ^ Magnification ^ Model ^ Type ^ NA ^ WD (mm) ^ Reference ^ | + | ^ Magnification ^ Model ^ Immersion ^ NA ^ WD (mm) ^ Reference ^ |
| - | | 25x | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420852-9973-000/Objective-LCI-Plan-Neofluar-25x-0.8-Imm-Corr-DIC-M27|LCI Plan-NeoFluar]] | Oil/Glyc/W | 0.8 | 0.21 | 420852-9973-000 | | + | | 25x | LCI Plan-NeoFluar Imm Corr DIC | Oil/Glyc/W | 0.8 | 0.21 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420852-9973-000/Objective-LCI-Plan-Neofluar-25x-0.8-Imm-Corr-DIC-M27|420852-9973-000]] | |
| - | | 40x | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420660-9970-000/Objective-Plan-Apochromat-40x-0.95-Corr-M27|Plan-Apochromat]] | Dry | 0.95 | 0.25 | 420660-9970-000 ^ | + | | 40x | Plan-Apochromat Corr | Air | 0.95 | 0.25 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420660-9970-000/Objective-Plan-Apochromat-40x-0.95-Corr-M27|420660-9970-000]] | |
| ==== Emission Filtersets ==== | ==== Emission Filtersets ==== | ||
| ^ Setting ^ Transmission ^ | ^ Setting ^ Transmission ^ | ||
| Line 69: | Line 70: | ||
| | 10: 445 | 422-477 nm | | | 10: 445 | 422-477 nm | | ||
| + | ==== Camera ==== | ||
| + | ^ Model ^ Frame Size ^ Pixel Size (µm) ^ Quantum Efficiency ^ | ||
| + | | [[https://www.photometrics.com/wp-content/uploads/2019/10/Evolve512-Datasheet.pdf|Photometrics Evolve 512 EMCCD]] | 512 x 512 | 16 x 16 | > 90% | | ||
| ===== Microscope Turn On Procedure ===== | ===== Microscope Turn On Procedure ===== | ||
| * Check that the main power supply switch is on (should be on by default) | * Check that the main power supply switch is on (should be on by default) | ||
3i_marianas_sdc.txt · Last modified: 2024/04/01 23:50 by bioimaging
Page Tools
