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3i_marianas_sdc [2019/12/31 18:17] bioimaging |
3i_marianas_sdc [2020/01/01 16:38] bioimaging [Microscope Turn On Procedure] |
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* **Microscope**: [[http://www.zeiss.com/microscopy/en_de/products/light-microscopes/axio-observer-for-biology.html|Zeiss Axio Observer]] | * **Microscope**: [[http://www.zeiss.com/microscopy/en_de/products/light-microscopes/axio-observer-for-biology.html|Zeiss Axio Observer]] | ||
- | * **Confocal scanner**: [[http://www.andor.com/microscopy_systems/peripherals/confocal_scanners/|Yokogawa CSU-x1]] | + | * **Confocal scanner**: [[https://www.yokogawa.com/eu/solutions/products-platforms/life-science/spinning-disk-confocal/csu-x1-confocal-scanner-unit/|Yokogawa CSU-X1]] |
- | * **Camera**: [[http://www.photometrics.com/products/emccdcams/evolve/512.php|Evolve 512 EMCCD]] | + | * **Camera**: [[https://www.photometrics.com/wp-content/uploads/2019/10/Evolve512-Datasheet.pdf|Evolve 512 EMCCD]] |
- | {{warning.jpg?20|}} Data files older than **1 month** will be automatically deleted on this system, please copy your data to the iMM server using the desktop link. | + | {{:warning.png?25|}} Data files older than **1 month** will be automatically deleted on this system, please copy your data to the iMM server using the desktop link. |
===== System components ===== | ===== System components ===== | ||
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==== Objectives ==== | ==== Objectives ==== | ||
- | ^ Magnification ^ Model ^ Type ^ NA ^ WD (mm) ^ | + | ^ Magnification ^ Model ^ Type ^ NA ^ WD (mm) ^ Reference ^ |
- | | 20x | [[https://www.micro-shop.zeiss.com/?s=129353259d52f12&l=en&p=uk&f=o&a=v&m=s&id=420650-9901-000|Plan-Apochromat]] | Dry | 0.8 | 0.55 | | + | | 20x | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420650-9902-000/Objective-Plan-Apochromat-20x-0.8-M27#|Plan-Apochromat]] | Dry | 0.8 | 0.55 | 420650-9902-000 | |
- | | 63x | [[https://www.micro-shop.zeiss.com/?s=3407786617d462&l=en&p=uk&f=o&a=v&m=s&id=420782-9900-000|Plan-Apochromat]] | Oil | 1.40 | 0.19 | | + | | 63x | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420780-9900-000/Objective-Plan-Apochromat-63x-1.4-Oil-M27|Plan-Apochromat]] | Oil | 1.40 | 0.19 | 420780-9900-000 | |
- | | 100x | [[https://www.micro-shop.zeiss.com/?s=129353259d52f12&l=en&p=uk&f=o&a=v&m=s&id=420790-9901-000|Plan-Apochromat]] | Oil | 1.40 | 0.17 | | + | | 100x | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420790-9901-000/Objective-Plan-Apochromat-100x-1.40-Oil-M27|Plan-Apochromat]] | Oil | 1.40 | 0.17 | 420790-9901-000 | |
**Upon request:** | **Upon request:** | ||
- | ^ Magnification ^ Model ^ Type ^ NA ^ WD (mm) ^ | + | ^ Magnification ^ Model ^ Type ^ NA ^ WD (mm) ^ Reference ^ |
- | | 25x | [[https://www.micro-shop.zeiss.com/?s=129353259d52f12&l=en&p=uk&f=o&a=v&m=s&id=420852-9972-000|LCI Plan-NeoFluar]] | Oil/Glyc/W | 0.8 | 0.21 | | + | | 25x | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420852-9973-000/Objective-LCI-Plan-Neofluar-25x-0.8-Imm-Corr-DIC-M27|LCI Plan-NeoFluar]] | Oil/Glyc/W | 0.8 | 0.21 | 420852-9973-000 | |
- | | 40x | [[https://www.micro-shop.zeiss.com/?s=129353259d52f12&l=en&p=uk&f=o&a=v&m=s&id=420660-9970-000|Plan-Apochromat]] | Dry | 0.95 | 0.25 | | + | | 40x | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420660-9970-000/Objective-Plan-Apochromat-40x-0.95-Corr-M27|Plan-Apochromat]] | Dry | 0.95 | 0.25 | 420660-9970-000 ^ |
==== Emission Filtersets ==== | ==== Emission Filtersets ==== | ||
^ Setting ^ Transmission ^ | ^ Setting ^ Transmission ^ | ||
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* Start the **SlideBook** software | * Start the **SlideBook** software | ||
- | **Warning**: If you need to change the stage adaptor, please contact the <html><a href="/facility/bioimaging/doku.php?id=about">Bioimaging Facility</a> (<b>imm-bioimaging@medicina.ulisboa.pt</b> | <img src="/facility/bioimaging/lib/exe/fetch.php?media=telephone_icon.gif" width=15> 47316</html>) | + | **Warning**: If you need to change the stage adaptor, please contact the <html><a href="/facility/bioimaging/doku.php?id=about">Bioimaging Unit</a> (<b>imm-bioimaging@medicina.ulisboa.pt</b> | <img src="/facility/bioimaging/lib/exe/fetch.php?media=phone_neg.png" width=15> 47222</html>) |
===== Microscope Turn Off Procedure ===== | ===== Microscope Turn Off Procedure ===== |