pt en

PTDC/104787/2008; PTDC/099142/2008; PTDC/69493/2006; PTDC/69937/2006

  • Isothermal Titration Calorimeter

    Isothermal Titration Calorimeter.jpg
    GE Healthcare
    Isothermal Titration Calorimeter

    Isothermal Titration Calorimetry (ITC) is used for measuring biomolecular interactions. ITC simultaneously determines all binding parameters (n, K, ∆H and ΔS) in a single experiment – information that cannot be obtained from any other method.

    When substances bind, heat is either generated or absorbed. ITC is a thermodynamic technique that directly measures the heat released or absorbed during a biomolecular binding event. Measurement of this heat allows accurate determination of binding constants (KB), reaction stoichiometry (n), enthalpy (∆H) and entropy (ΔS), thereby providing a complete thermodynamic profile of the molecular interaction in a single experiment.Interactions between any two molecules can be studied with ITC, including:

    • Protein-small molecule
    • Protein-protein
    • Target-drug
    • Enzyme-inhibitor
    • Antibody-antigen
    • Protein-DNA
    • Protein-lipid
    • Small molecule-small molecule

    Applications include:

    • Characterization of molecular interactions of small molecules, proteins, antibodies, nucleic acids, lipids and other biomolecules;
    • Lead optimization;
    • Enzyme kinetics;
    • Assessment of the effect of molecular structure changes on binding mechanisms.
    • Assessment of biological activity.
    Open this equipment separately

This site uses cookies. Some of the cookies we use are essential for parts of the site to operate and have already been set.
You may delete and block all cookies from this site, but parts of the site will not work.