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PTDC/104787/2008; PTDC/099142/2008; PTDC/69493/2006; PTDC/69937/2006

  • Isothermal Titration Calorimeter

    Isothermal Titration Calorimeter.jpg
    GE Healthcare
    VP-ITCell
    FIM2145
    P2B45
    Isothermal Titration Calorimeter

    Isothermal Titration Calorimetry (ITC) is used for measuring biomolecular interactions. ITC simultaneously determines all binding parameters (n, K, ∆H and ΔS) in a single experiment – information that cannot be obtained from any other method.

    When substances bind, heat is either generated or absorbed. ITC is a thermodynamic technique that directly measures the heat released or absorbed during a biomolecular binding event. Measurement of this heat allows accurate determination of binding constants (KB), reaction stoichiometry (n), enthalpy (∆H) and entropy (ΔS), thereby providing a complete thermodynamic profile of the molecular interaction in a single experiment.Interactions between any two molecules can be studied with ITC, including:

    • Protein-small molecule
    • Protein-protein
    • Target-drug
    • Enzyme-inhibitor
    • Antibody-antigen
    • Protein-DNA
    • Protein-lipid
    • Small molecule-small molecule

    Applications include:

    • Characterization of molecular interactions of small molecules, proteins, antibodies, nucleic acids, lipids and other biomolecules;
    • Lead optimization;
    • Enzyme kinetics;
    • Assessment of the effect of molecular structure changes on binding mechanisms.
    • Assessment of biological activity.
    Open this equipment separately

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