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Saldanha, Carlota
Lab

Blood Cells Recruitment & Inflammation

The vascular wall stays at the cornerstone of inflammation controlling the participation and recruitment of circulating blood cells. Our research is focused on the molecular and cellular processes occurring at blood-vessel wall interface in the context of inflammation. To address this, we conjugate molecular and cellular biology approaches with microcirculation studies, such as high-speed intravital microscopy imaging of in vivo animal models.
By using rat, mice and zebrafish models, our research aims at understanding the mechanisms that govern leukocyte recruitment and cell-cell interaction in inflammation. We are currently addressing the role of specific inflammatory mediators, in modulating blood-vessel wall interaction in inflammation and the mechanisms that regulate neutrophil-induced monocyte recruitment during the inflammatory response. We research on the role of erythrocyte as a pro-active blood component on inflammation.
Based on these studies, we collaborate at developing theoretical simulation models of phenomena occurring at the level of leukocyte-vascular wall interface, particularly related to inflammatory processes.
Finally, we are also collaborating with a start-up biotech company in the development of new methodologies for the determination of hemorheologic parameters.

  • Research Areas

    • Inflammation
    • Leukocyte-endothelium interaction / Microcirculation
    • Hemorheology
    • Non-Neuronal Cholinergic System
  • Research Team

    • Ongoing Research Projects

      • 2013/2016 "Effects of Timolol on Biochemical and Hemorheological Properties of Erythrocytes from Glaucoma Patients". In collaboration with Carlos Marques-Neves FMUL Professor, ISAMB, CECU Coordenator, ALM Clinical Director
      • 2014/2016 Perfis da Agregação Eritrocitária e da IL-8 em sobreviventes de enfarte agudo de miocárdio. Dr. Daniel Ferreira (Hospital da Luz), Coordinator: C. Saldanha
      • 2013/2015 Evaluation of inflammatory and adaptative immunity imbalance to improve prognosis of ischemic cardiovascular diseases, Liga dos Amigos do Hospital de Santa Marta/2012. Coordinator: Patrícia Napoleão.
      • 2013/2015 Contribution of circulating microRNA to improve prognosis of ischemic cardiovascular diseases, Liga dos Amigos do Hospital de Santa Marta/2012. Coordinator: Patricia Napoleão
      • 2012/2015 Mathematical and computational modeling of human physiology EXCL/Mat-NAN/0114/2012 A. Sequeira (CEMAT/IST).
      • 2012/2015 Evaluation of inflammation profiles in septic patients – contribution to multiple cross prognostic biomarkers. Coordinator: Carlota Saldanha.
      • 2011/2013 Functional characterization of the neutrophil in alcoholic and non-alcoholic steatohepatiti. Coordinator: Ângelo Calado.
      • 2011/2013 Functional characterization of the neutrophil in alcoholic and non-alcoholic steatohepatitis. Coordinator: A. Calado.
      • 2009/2013 In vivo imaging of inflammation in zebrafish: the role of chemokine system in the neutrophil and macrophage migration, Sofia de Oliveira, PhD Fellowship SFRH/BD/62674/2009, FCT. Coordinator: A. Calado.
      • 2009/2013 Interrelationship of circulating haemostatic, hemorheologic and inflammatory markers in acute myocardial infarction – a longitudinal approach. Coordinator: Carlota Saldanha. Patrícia Napoleão Post-Doc Fellowship SFRM/BPD/63908/2009, FCT.
      • 2009/2013 Regulatory interactions between the metabolic sensors SIRT1 and AMPK in modulating PPARalpha-linked functions in liver in health and disease, Co-Coordinator: Carlota Saldanha. Marta Silvestre PhD Fellowship SFRH /BD/62927/2009, FCT.
      • Previous projects
      • 2009/2011 Development and validation of vaso-occlusion early predictors in a a mendelian model of vascular disease, FCT. Coordinator: João Lavinha, INSA. PIC/IC/83084/2007
      • 2009/2011 Cardiovascular risk and inflammation- contribution to the etiopathogenesis of atherosclerosis and cardiovascular disease, FCT. Coordinator: João Eurico, IMM, FML, UL. PIC/IC/82920/2007.
      • 2009/  Hemodisc: hemorheology on a disc. Project PTDC/SAU-ENB/112931/2009 in submission to FCT. Principal Investigator: Carlota Saldanha.
      • 2009/  Inflammation in zebrafish: in vivo study of the role of chemokine networks in neutrophil and macrophage migration. Project PTDC/SAU-IMU/111497/2009 in submission to FCT. Principal Investigator: Angelo Calado.
      • 2009/  Neutrophil in canine Leishmaniasis: uncovering cellular mechanisms and potential therapeutic opportunities. Project PTDC/CVT/111719/2009 in submission to FCT. Principal investigator: Henrique Sobral do Rosário.
      • 2007/2009  Fibrinogen-dependent signalling in microvascular blood cell function – implications of inflammation and cardiovascular disease, FCT (PTDC/SAU-OSM/73449/2006)
      • 2007/2009 Multiscale Mathematical Modelling in Biomedicine, FCT (PTDC/MAT/68166/2006)
      • 2007/ 2012 The relationship of circulating biomarkers of apoptosis and endothelial function with the plaque composition using VH IVUS, FCT. Coordinator: Teresa Pinheiro, Grupo de Estudos Biomédicos, Instituto Tecnológico e Nuclear (ITN). FCT project PIC/IC/82734/2007
      • 2007/2009 Identificação de um marcador de interacção eficaz na terapêutica de doença de Alzheimer, Fundação Merck Shape & Dohme
      • 2007/2011 Cardiovascular risk and inflammation- contribution to the etiopathogenesis of atherosclerosis and cardiovascular disease, FCT (PIC/IC/82920/2007)
      • 2007/2011 Development and validation of vaso-occlusion early predictors in a a mendelian model of vascular disease, FCT (PIC/IC/83084/2007)
      • 2006/  Effects of long-term therapy with acetylcholinesterase and butyrilcholinesterase inhibitors in Alzheimer disease
      • 2006/  Factores preditivos de lesão endotelial e cardiovascular no prognóstico da esclerose sistémica e sua correlação com a terapêutica
      • 2006/  Multiscale Mathematical Modelling in Biomedicine. Foundation for Science and Technology, Portugal – Project PTDC/ MAT/ 68166/2006
      • 2005/  Molecular Medicine – from genome to clinical practice (Team member; responsible for selection of a high-speed intravital confocal microscope). Foundation for Science and Technology, Portugal – Project REEQ/650/SAU/2005.
      • 2002/2006 Mathematical and Numerical Modelling in Haemodynamics – HaeModel. EU-RTN European Research Training Network - HPRN-CT 2002-00270.
      • 2001/2004 Mathematical and Numerical Modelling of the Human Cardiovascular System. Foundation for Science and Technology, Portugal - Project POCTI/MAT/41898/ 2001
      • 1998/  Acetylcholinesterase and chronic venous diseases. HPV
    • Prizes

      • Napoleão, P. (2014) - "Hemorreologia, Hemostase e Inflamação na Patologia Vascular – Da investigação à prática clínica", Lisbon, Portugal, Award of SPHM-SPACV/Biosaúde.
      • de Oliveira, S., López-Muñoz, A., Candel, S., Calado, A. and Mulero, V. (2013) "Early ATP and calcium signals modulate zebrafish acute inflammatory response via Duox1 activation and hydrogen peroxide release". ZDM6 – Zebrafish Disease Models 6, Murcia, Spain. 14th-17th July. Awarded prize for Best Oral Presentation in ZDM6.
      • Napoleão, P. (2012) Award SPHM-SPACV/BAYER para Best Clinical Work presented at Reunião Conjunta da Sociedade Portuguesa de Hemorreologia e Microcirculação e Núcleo de Biologia Vascular da Sociedade Portuguesa de Angiologia e Cirurgia Vascular "Hemorreologia, Hemostase e Inflamação na Patologia Vascular – Da investigação à prática clínica"
    • Selected Publications

      • de Oliveira S, López-Muñoz A, Candel S, Pelegrín P, Calado A, Mulero V. (2014) ATP Modulates Acute Inflammation In Vivo through Dual Oxidase 1-Derived H2O2 Production and NF-κB Activation. J.Immunol. May 19. pii: 1302902. [Epub ahead of print]
      • Candel S, de Oliveira S, López-Muñoz, García-Moreno V, Espín-Palazón R, Tyrkalska SD, Cayuela ML, Renshaw SA, Corbalán-Vélez R, Vidal-Abarca I, Tsai HJ, Meseguer J, Sepulcre MP, Mulero V. (2014) Tnfa signaling through tnfr2 protects skin against oxidative stress-induced inflammation. PLoS Biology May 6;12(5) (DOI: 10.1371/journal.pbio.1001855. eCollection 2014)
      • Saldanha C, Freitas T, Lopes de Almeida JP, Silva-Herdade AS. (2014) Signal transduction pathways in erythrocyte nitric oxide metabolism under high fibrinogen levels. Korea-Australia Rheology Journal, Vol.26, No.2, pp.217-223 (DOI: 10.1007/s13367-014-0024-2)
      • Saldanha C, Silva-Herdade AS. (2014) The Ubiquity Nature of Acetylcholine, Clinical & Experimental Pharmacology (in press)
      • Teixeira P, Napoleão P, Saldanha C. (2014) S-Nitrosoglutathione Efflux in the Erythrocyte, Clinical Hemorheology and Microcirculation (in press)
      • Silva-Herdade AS, Freitas T, Almeida JP, Saldanha C.Erythrocyte deformability and nitric oxide mobilization under pannexin-1 and PKC dependence. Clin Hemorheol Microcirc. 2014 Mar 4. [Epub ahead of print]
      • Saldanha, C., Lopes de Almeida, JP, Silva-Herdade, AS. (2014) Application of a nitric oxide sensor in biomedicine. Biosensors 4, 1-17. (DOI:10.3390/bios4010001))
      • de Almeida, V.V., Calado, A., Silva-Herdade, A.S., Rosário, H.S., Saldanha, C. (2014) An in vitro study on the modulation of the neutrophil adhesive behavior by soluble fibrinogen. Clin Hemorheol Microcirc 1, 56(1) :47-56. (DOI 10.3233/CH-121662)
      • de Oliveira, S., Reyes-Aldasoro, C.C., Candel, S., Renshaw, S.A., Mulero, V., Calado, A. (2013) Cxcl8 (Interleukin-8) mediates neutrophil recruitment and behavior in the zebrafish inflammatory response. J.Immunol. 15; 190(8): 4349-59.
      • Saldanha, C., Teixeira, P., Santos-Freitas, T, Napoleão, P. (2013) Timolol modulates erythrocyte nitric oxide bioavailability. J Clin Exp Ophalmol 4 (3): 1-3.
      • Barvitenko, N.N., Aslam, M., Filosa, J., Matteucci, E., Nikinmaa, M., Pantaleo, A., Saldanha, C., Baskurt, O.K. (2013) Tissue oxygen demand in regulation of the behavior of the cells in the vasculature. Microcirculation 20(6): 484-501
      • Silva-Herdade AS, Saldanha C. (2013) Effects of acetylcholine on an animal model of inflammation. Clin Hemorheol Microcirc. 53(1), 195-202.
      • Napoleão, P., Selas M., Freixo C., Mota Carmo M., Viegas-Crespo A.M., Ferreira R.C., Pinheiro T. (2013) T lymphocytes alterations are associated with oxidized LDL, troponin T, white blood cells and C-reactive protein during acute myocardial infarction, Clin Hemorheol Microcirc. 55(3):349-58.
      • Saldanha, C. (2013) Fibrinogen interaction with the red blood cell membrane. Clin Hemorheol Microcirc., 53(1-2):39-44
      • de Almeida, V.V., Silva-Herdade, A.S., Calado, A., Rosário, H.S., Saldanha, C. (2012) Fibrinogen modulates leukocyte recruitment in vivo during the acute inflammatory response. Clin Hemorheol Microcirc. 2012 Dec 27. [Epub ahead of print]
      • J. Galindo-Villegas, D. García-Moreno, S. de Oliveira, J. Meseguer, V. Mulero, (2012) Regulation of immunity and disease resistance by commensal microbes and chromatin modifications during zebrafish development.” Proc Natl Acad Sci U S A., Sep 25, 109(39), 2605-2614
      • de Almeida VV, Calado A, Rosário HS, Saldanha C. (2012) Differential effect of soluble fibrinogen as a neutrophil activator. Microvasc Res. 83(3):332-6.
      • C. Saldanha, (2012) Fibrinogen interaction with the red blood cell membrane. Clin Hemorheol Microcirc., Sep 7. [Epub ahead of print]
      • C. Saldanha, T. Freitas, JP. Almeida, (2012) Fibrinogen effects on erythrocyte nitric oxide mobilization in presence of acetylcholine. Life Sci.91(21-22), 1017-22.
      • P. de Oliveira, AQ Gomes, TR Pacheco, V Vitorino de Almeida, C Saldanha, A Calado, (2012) Cell-specific regulation of acetylcholinesterase expression under inflammatory conditions. Clin Hemorheol Microcirc. 51(2), 129-37.
      • JP Lopes de Almeida, T Freitas-Santos, C Saldanha, (2012) Erythrocyte deformability dependence on band 3 protein in an in-vitro model of hyperfibrinogenemia. Clin Hemorheol Microcirc, 50(3), 213-9.
      • Lopes de Almeida, JP, Freitas-Santos, T, Saldanha, C. (2011) Evidence that the degree of band 3 phosphorylation modulates human erythrocytes nitric oxide efflux - in vitro model of hyperfibrinogenemia. Clinical Hemorheology and Microcirculation 49 (1), 407-16.
      • Saldanha, C, Lopes de Almeida, JP. (2011) Erythrocyte as a link between basic and clinical research. Clinical Hemorheology and Microcirculation 49 (1), 463-72.
      • Moura, RA, Cascao, R, Perpetuo, I, Canhao, H, Vieira-Sousa, E, Mourao, AF, Rodrigues, AM, Polido-Pereira, J, Queiroz, MV, Rosario, HS, Souto-Carneiro, MM, Graca, L, Fonseca, JE. (2011) Cytokine pattern in very early rheumatoid arthritis favours B-cell activation and survival. Rheumatology 50 (2), 278-282
      • de Oliveira, S, de Almeida, VV, Calado, A, Rosário, HS, Saldanha, C. (2011) Integrin-associated protein (CD47) is a putative mediator for soluble fibrinogen interaction with human red blood cells membrane. Integrin Biochim Biophys Acta 1818 (3), 481-490
      • Santos, MJ, Pedro, LM, Canhão, H, Fernandes e Fernandes, J, Canas da Silva, J, Fonseca, JE, Saldanha C. (2011) Hemorheological parameters are related to subclinical atherosclerosis in systemic lupus erythematosus and rheumatoid arthritis patients. Atherosclerosis 219(2), 821-6.
      • Silva-Herdade, AS, Saldanha, C. (2011) Hemorheological effects of valsartan in L-NAME induced hypertension in rats. The Open Circ Vasc J 4, 1-5.
      • Lopes de Almeida JP, Freitas-Santos, T, Saldanha C. (2011) Erythrocyte deformability dependence on Band 3 protein in an in vitro model of hyperfibrinogenemia. Clin Hemorh Microc 44, 1-6.
      • Lopes de Almeida, JP, de Oliveira, S, Saldanha, C. (2011) Erythrocyte as a Biological Sensor. Clin Hemorh Microc 48, 1-20.
      • Saldanha, C, Lopes de Almeida, JP, Freitas, T, Oliveira, S, Silva-Herdade, AS. (2010) Erythrocyte deformability responses to shear stress under external and internal stimuli influences. Series on Biomechanics 25, 54-60.
      • Lopes de Almeida J.P., Saldanha, C. (2010) Dithiothreitol revisited in red cells: a new head for an old hat. Clin Hemorheol Microcirc. 46, 51-56.
      • Martin Martins, J., Vale, S., Ferreira, F., Fagundes, M.J., Carmo, I., Saldanha, C., Martins e Silva, J. (2010) Plasma corticotropin releasing hormone during the feeling of induced emotions. Neuro Endocrinol Lett. 31, 250-5.
      • de Almeida, J.P., Saldanha, C. (2010) Nonneuronal cholinergic system in human erythrocytes: biological role and clinical relevance. J Membr Biol. 234(3), 227-34.
      • Santos, N.C., Silva, J.M., Freitas, T., Doroana, M., Duarte, N., Tavares, L., Antunes, F., Saldanha, C. (2010) Blood cell membrane fluidity and intracellular Ca2+ changes in antiretroviral-naïve and -treated HIV-1-infected patients. The Scientific World Journal. 10, 350-5.
      • de Oliveira, S., Saldanha, C. (2010) An overview about erythrocyte membrane. Clin Hemorheol Microcirc. 44, 63-74.
      • Cascão R, Moura RA, Perpétuo I, Canhão H, Vieira-Sousa E, Mourão AF, Rodrigues AM, Polido-Pereira J, Queiroz MV, Rosário HS, Souto-Carneiro MM, Graca L, Fonseca JE (2010) Identification of a cytokine network sustaining neutrophil and Th17 activation in untreated early rheumatoid arthritis. Arthritis Res Ther 12, R196.
      • Cascão R, Rosário HS, Souto-Carneiro MM, Fonseca JE. (2010) Neutrophils in rheumatoid arthritis: More than simple final effectors. Autoimmun Rev 2010 9, 531-535.
      • Cascão, R., Rosário, H.S., Fonseca, J.E. (2009) Neutrophils: warriors and commanders in immune mediated inflammatory diseases. Acta Reumatol Port 34 (2B), 313-326.
      • Lopes de Almeida, J.P., Carvalho, F.A., Silva-Herdade, A.S., Santos-Freitas, T., Saldanha, C. (2009) Redox thiol status plays a central role in the mobilization and metabolism of nitric oxide in human red blood cells. Cell Biol Int 33 (3), 268-275.
      • Sequeira, A., Artoli, A.M., Silva-Herdade, A.S. Saldanha, C. (2009) Leukocytes dynamics in microcirculation under shear-thining blood flow. Computers & Mathematics with Applications 58 (5), 1035-1044.
      • Carvalho, F.A., de Almeida, J.P., Freitas-Santos, T., Saldanha, C. (2009)Modulation of erythrocyte acetylcholinesterase activity and its association with G protein-band 3 interactions. J Membr Biol 228(2), 89-97.
      • Artoli AM, Sequeira A, Silva-Herdade AS, Saldanha C (2008). Localized hydrodynamics of clustering leukocytes. Clin Hemorheol Microcirc 39 (1-4), 375-380.
      • Carvalho FA, Almeida JP, Fernandes IO, Freitas-Santos T, Saldanha C. (2008) Non-neuronal cholinergic system and signal transduction pathways mediated by band 3 in red blood cells. Clin Hemorheol Microcirc 40:207-227.
      • de Oliveira S, Silva-Herdade AS, Saldanha C. (2008) Modulation of erythrocyte deformability by PKC activity. Clin Hemorheol Microcirc 39 (1-4), 363-373.
      • Almeida JP, Carvalho FA, Freitas T, Saldanha C. (2008) Modulation of hemorheological parameters by the erythrocyte redox thiol status. Clin Hemorheol Microcirc 40, 99-111.
      • Vieira CR, Castanho M, Saldanha C, Santos NC. (2008) Enfuvirtide effects on human erythrocytes and lymphocytes functional properties. J Pept Sci 14, 448-454.
      • Canhão H, Fonseca JE, Caetano-Lopes J, Saldanha C, Queiroz MV. (2008) Assessment of laboratory measurements and -308 TNFalpha gene promoter polymorphisms in normal bone mineral density. Clin Rheumatol 27, 301-307.

      Full publications list

      2014

      • de Oliveira S, López-Muñoz A, Candel S, Pelegrín P, Calado A, Mulero V. (2014) ATP Modulates Acute Inflammation In Vivo through Dual Oxidase 1-Derived H2O2 Production and NF-κB Activation. J.Immunol. May 19. pii: 1302902. [Epub ahead of print]
      • Candel S, de Oliveira S, López-Muñoz, García-Moreno V, Espín-Palazón R, Tyrkalska SD, Cayuela ML, Renshaw SA, Corbalán-Vélez R, Vidal-Abarca I, Tsai HJ, Meseguer J, Sepulcre MP, Mulero V. (2014) Tnfa signaling through tnfr2 protects skin against oxidative stress-induced inflammation. PLoS Biology May 6;12(5) (DOI: 10.1371/journal.pbio.1001855. eCollection 2014)
      • Saldanha C, Freitas T, Lopes de Almeida JP, Silva-Herdade AS. (2014) Signal transduction pathways in erythrocyte nitric oxide metabolism under high fibrinogen levels. Korea-Australia Rheology Journal, Vol.26, No.2, pp.217-223 (DOI: 10.1007/s13367-014-0024-2)
      • Saldanha C, Silva-Herdade AS. (2014) The Ubiquity Nature of Acetylcholine, Clinical & Experimental Pharmacology (in press)
      • Teixeira P, Napoleão P, Saldanha C. (2014) S-Nitrosoglutathione Efflux in the Erythrocyte, Clinical Hemorheology and Microcirculation (in press)
      • Silva-Herdade AS, Freitas T, Almeida JP, Saldanha C.Erythrocyte deformability and nitric oxide mobilization under pannexin-1 and PKC dependence. Clin Hemorheol Microcirc. 2014 Mar 4. [Epub ahead of print]
      • Saldanha, C., Lopes de Almeida, JP, Silva-Herdade, AS. (2014) Application of a nitric oxide sensor in biomedicine. Biosensors 4, 1-17. (DOI:10.3390/bios4010001))
      • de Almeida, V.V., Calado, A., Silva-Herdade, A.S., Rosário, H.S., Saldanha, C. (2014) An in vitro study on the modulation of the neutrophil adhesive behavior by soluble fibrinogen. Clin Hemorheol Microcirc 1, 56(1) :47-56. (DOI 10.3233/CH-121662)
      • Silva-Herdade AS, Freitas T, Almeida JP, Saldanha C.Erythrocyte deformability and nitric oxide mobilization under pannexin-1 and PKC dependence. Clin Hemorheol Microcirc. 2014 Mar 4. [Epub ahead of print]
      • Saldanha, C., Lopes de Almeida, JP, Silva-Herdade, AS. (2014) Application of a nitric oxide sensor in biomedicine. Biosensors 4, 1-17.
      • de Almeida, V.V., Calado, A., Silva-Herdade, A.S., Rosário, H.S., Saldanha, C. (2014) An in vitro study on the modulation of the neutrophil adhesive behavior by soluble fibrinogen. Clin Hemorheol Microcirc 1, 56(1) :47-56.

      2013

      • de Oliveira, S., Reyes-Aldasoro, C.C., Candel, S., Renshaw, S.A., Mulero, V., Calado, A. (2013) Cxcl8 (Interleukin-8) mediates neutrophil recruitment and behavior in the zebrafish inflammatory response. J.Immunol. 15; 190(8): 4349-59.
      • Saldanha, C., Teixeira, P., Santos-Freitas, T, Napoleão, P. (2013) Timolol modulates erythrocyte nitric oxide bioavailability. J Clin Exp Ophalmol 4 (3): 1-3.
      • Barvitenko, N.N., Aslam, M., Filosa, J., Matteucci, E., Nikinmaa, M., Pantaleo, A., Saldanha, C., Baskurt, O.K. (2013) Tissue oxygen demand in regulation of the behavior of the cells in the vasculature. Microcirculation 20(6): 484-501
      • Silva-Herdade AS, Saldanha C. (2013) Effects of acetylcholine on an animal model of inflammation. Clin Hemorheol Microcirc. 53(1), 195-202.
      • Napoleão, P., Selas M., Freixo C., Mota Carmo M., Viegas-Crespo A.M., Ferreira R.C., Pinheiro T. (2013) T lymphocytes alterations are associated with oxidized LDL, troponin T, white blood cells and C-reactive protein during acute myocardial infarction, Clin Hemorheol Microcirc. 55(3):349-58.
      • Saldanha, C. (2013) Fibrinogen interaction with the red blood cell membrane. Clin Hemorheol Microcirc., 53(1-2):39-44

      2012

      • de Almeida, V.V., Silva-Herdade, A.S., Calado, A., Rosário, H.S., Saldanha, C. (2012) Fibrinogen modulates leukocyte recruitment in vivo during the acute inflammatory response. Clin Hemorheol Microcirc. 2012 Dec 27. [Epub ahead of print]
      • J. Galindo-Villegas, D. García-Moreno, S. de Oliveira, J. Meseguer, V. Mulero, (2012) Regulation of immunity and disease resistance by commensal microbes and chromatin modifications during zebrafish development.” Proc Natl Acad Sci U S A., Sep 25, 109(39), 2605-2614
      • de Almeida VV, Calado A, Rosário HS, Saldanha C. (2012) Differential effect of soluble fibrinogen as a neutrophil activator. Microvasc Res. 83(3):332-6.
      • C. Saldanha, (2012) Fibrinogen interaction with the red blood cell membrane. Clin Hemorheol Microcirc., Sep 7. [Epub ahead of print]
      • Lopes de Almeida JP, Oliveira S, Saldanha C. (2012) Erythrocyte as a biological sensor. Clin Hemorheol Microcirc. 51(1), 1 -20.
      • C. Saldanha, T. Freitas, JP. Almeida, (2012) Fibrinogen effects on erythrocyte nitric oxide mobilization in presence of acetylcholine. Life Sci., Jul 11. [Epub ahead of print]
      • de Oliveira, P Gomes, AQ, Pacheco, TR, Vitorino de Almeida, V, Saldanha, C, Calado, A (2012) Cell-specific regulation of acetylcholinesterase expression under inflammatory conditions. Clin Hemorheol Microcirc. 51(2), 129-37.
      • JP Lopes de Almeida, T Freitas-Santos, C Saldanha, (2012) Erythrocyte deformability dependence on band 3 protein in an in-vitro model of hyperfibrinogenemia. Clin Hemorheol Microcirc, 50(3), 213-9.

      2011

      • Lopes de Almeida, JP, Freitas-Santos, T, Saldanha, C. (2011) Evidence that the degree of band 3 phosphorylation modulates human erythrocytes nitric oxide efflux - in vitro model of hyperfibrinogenemia. Clinical Hemorheology and Microcirculation 49 (1), 407-16.
      • Saldanha, C, Lopes de Almeida, JP. (2011) Erythrocyte as a link between basic and clinical research. Clinical Hemorheology and Microcirculation 49 (1), 463-72. 
      • Moura, RA, Cascao, R, Perpetuo, I, Canhao, H, Vieira-Sousa, E, Mourao, AF, Rodrigues, AM, Polido-Pereira, J, Queiroz, MV, Rosario, HS, Souto-Carneiro, MM, Graca, L, Fonseca, JE. (2011) Cytokine pattern in very early rheumatoid arthritis favours B-cell activation and survival. Rheumatology 50 (2), 278-282
      • de Oliveira, S, de Almeida, VV, Calado, A, Rosário, HS, Saldanha, C. (2011) Integrin-associated protein (CD47) is a putative mediator for soluble fibrinogen interaction with human red blood cells membrane. Biochim Biophys Acta 1818 (3), 481-490
      • Santos, MJ, Pedro, LM, Canhão, H, Fernandes e Fernandes, J, Canas da Silva, J, Fonseca, JE, Saldanha C. (2011) Hemorheological parameters are related to subclinical atherosclerosis in systemic lupus erythematosus and rheumatoid arthritis patients. Atherosclerosis 219(2), 821-6.
      • Silva-Herdade, AS, Saldanha, C. (2011) Hemorheological effects of valsartan in L-NAME induced hypertension in rats. The Open Circ Vasc J 4, 1-5.
      • Lopes de Almeida JP, Freitas-Santos, T, Saldanha C. (2011) Erythrocyte deformability dependence on Band 3 protein in an in vitro model of hyperfibrinogenemia. Clin Hemorh Microc 44, 1-6.
      • Lopes de Almeida, JP, de Oliveira, S, Saldanha, C. (2011) Erythrocyte as a Biological Sensor. Clin Hemorh Microc 48, 1-20.

      2010

      • Saldanha, C, Lopes de Almeida, JP, Freitas, T, Oliveira, S, Silva-Herdade, AS. (2010) Erythrocyte deformability responses to shear stress under external and internal stimuli influences. Series on Biomechanics 25, 54-60.
      • Lopes de Almeida J.P., Saldanha, C. (2010) Dithiothreitol revisited in red cells: a new head for an old hat. Clin Hemorheol Microcirc. 46, 51-56.
      • Martin Martins, J., Vale, S., Ferreira, F., Fagundes, M.J., Carmo, I., Saldanha, C., Martins e Silva, J. (2010) Plasma corticotropin releasing hormone during the feeling of induced emotions. Neuro Endocrinol Lett. 31, 250-5.
      • de Almeida, J.P., Saldanha, C. (2010) Nonneuronal cholinergic system in human erythrocytes: biological role and clinical relevance. J Membr Biol. 234(3), 227-34.
      • Santos, N.C., Silva, J.M., Freitas, T., Doroana, M., Duarte, N., Tavares, L., Antunes, F., Saldanha, C. (2010) Blood cell membrane fluidity and intracellular Ca2+ changes in antiretroviral-naïve and -treated HIV-1-infected patients. The Scientific World Journal. 10, 350-5.
      • de Oliveira, S., Saldanha, C. (2010) An overview about erythrocyte membrane. Clin Hemorheol Microcirc. 44, 63-74.
      • Moura RA, Cascão R, Perpétuo I, Canhão H, Vieira-Sousa E, Mourão AF, Rodrigues AM, Polido-Pereira J, Queiroz MV, Rosário HS, Souto-Carneiro MM, Graca L, Fonseca JE (2010) Cytokine pattern in very early rheumatoid arthritis favours B-cell activation and survival. Rheumatology, 50(2), 278-282.
      • Cascão R, Moura RA, Perpétuo I, Canhão H, Vieira-Sousa E, Mourão AF, Rodrigues AM, Polido-Pereira J, Queiroz MV, Rosário HS, Souto-Carneiro MM, Graca L, Fonseca JE (2010) Identification of a cytokine network sustaining neutrophil and Th17 activation in untreated early rheumatoid arthritis. Arthritis Res Ther 12, R196.
      • Cascão R, Rosário HS, Souto-Carneiro MM, Fonseca JE. Neutrophils in rheumatoid arthritis: More than simple final effectors (2010) Autoimmun Rev 2010 9, 531-535.

      2009

      • Cascão, R., Rosário, H.S., Fonseca, J.E. (2009) Neutrophils: warriors and commanders in immune mediated inflammatory diseases. Acta Reumatol Port 34 (2B), 313-326. 
      • Lopes de Almeida, J.P., Carvalho, F.A., Silva-Herdade, A.S., Santos-Freitas, T., Saldanha, C. (2009) Redox thiol status plays a central role in the mobilization and metabolism of nitric oxide in human red blood cells. Cell Biol Int 33 (3), 268-275.
      • Sequeira, A., Artoli, A.M., Silva-Herdade, A.S. Saldanha, C. (2009) Leukocytes dynamics in microcirculation under shear-thining blood flow. Computers & Mathematics with Applications 58 (5), 1035-1044. 
      • de Almeida, J.P., Freitas-Santos, T., Saldanha, C. (2009) Fribrinogen-dependent signaling im microvascular erytrocyte finction: implications on nitric oxide efflux. J Membr Biol 231(1), 47-53
      • Carvalho, F.A., de Almeida, J.P., Freitas-Santos, T., Saldanha, C. (2009) Modulation of erythrocyte acetylcholinesterase activity and its association with G protein-band 3 interactions. J Membr Biol 228(2), 89-97.

      2008

      • Artoli AM, Sequeira A, Silva-Herdade AS, Saldanha C (2008) Localized hydrodynamics of clustering leukocytes. Clin Hemorheol Microcirc 39 (1-4), 375-380.
      • Carvalho FA, Almeida JP, Fernandes IO, Freitas-Santos T, Saldanha C. (2008) Non-neuronal cholinergic system and signal transduction pathways mediated by band 3 in red blood cells. Clin Hemorheol Microcirc 40:207-227.
      • de Oliveira S, Silva-Herdade AS, Saldanha C. (2008) Modulation of erythrocyte deformability by PKC activity. Clin Hemorheol Microcirc 39 (1-4), 363-373.
      • Almeida JP, Carvalho FA, Freitas T, Saldanha C. (2008) Modulation of hemorheological parameters by the erythrocyte redox thiol status. Clin Hemorheol Microcirc 40, 99-111.
      • Vieira CR, Castanho M, Saldanha C, Santos NC. (2008) Enfuvirtide effects on human erythrocytes and lymphocytes functional properties. J Pept Sci 14, 448-454.
      • Canhão H, Fonseca JE, Caetano-Lopes J, Saldanha C, Queiroz MV. (2008) Assessment of laboratory measurements and -308 TNFalpha gene promoter polymorphisms in normal bone mineral density. Clin Rheumatol 27, 301-307.

      2007

      • Artoli, A.M., Sequeira, A., Silva-Herdade, A.S., Saldanha, C. (2007) Leukocytes rolling and recruitment by endothelial cells: hemorheological experiments and numerical simulations. J Biomech. 40(15): 3493-502.
      • Gonçalves, S., Vasco Maria, A., Silva-Herdade, A.S., Martins e Silva, J., Saldanha, C. (2007) Milk enriched with phytosterols reduces plasma cholesterol levels in healthy and hypercholesterolemic subjects. Nutrition Research 27; 200-205.
      • Silva, A.S., Saldanha C., Martins e Silva, J. (2007) Effects of velnacrine maleate in the leukocyte-endothelial cell interactions in rat cremaster microcirculatory effects. Clin. Hemorheol. Microcirc. 36: 235-246.
      • Santos, S.C., Miguel, C., Domingues, I., Calado, A., Zhu, Z., Wu, Y. and Dias, S. (2007) VEGF and VEGFR-2 (KDR) internalization is required for endothelial recovery during wound healing. Exp. Cell Res. 313,1561-1574.
      • Santos, S.C.R., Vala, I., Miguel, C., Barata, J.P., Garção, P., Agostinho, P., Mendes, M., Coelho, A.V., Calado, A., Oliveira, C.R., Martins e Silva, J. and Saldanha, C. (2007) Expression and subcellular localization of a novel nuclear acetylcholinesterase protein. J. Biol. Chem. 282, 25597-25603.
      • Saldanha, C., Silva, A.S., Gonçalves, S., Martins e Silva, J. (2007) Modulation of erythrocyte hemorheological properties by band 3 phosphorylation and dephosphorylation. Clin Hemorheol and Microcirc. 36; 183-194.
      • Gonçalves, S., Santos, N.C., Martins-Silva, J., Saldanha, C. (2007) Fluorescence spectroscopy evaluation of fibrinogen-β-estradiol binding. J. Photochem. Photobiol. B-Biol. 86: 170-176.
      • Almeida, J.P. (2007) Circulating Acetylcholine Revisited: Physiological and Clinical Relevance. JEMSA – Journal of EMSA on Medical and Scientific Affairs 2007, 26-32.

      2006-…

      • Gonçalves S, Santos NC, Martins-Silva J, Saldanha C. (2006) Fluorescence spectroscopy evaluation of fibrinogen-beta-estradiol binding. J Photochem Photobiol B. 86(2), 170-6.
      • Martins-Silva J, Santos NC, Doroana M, Duarte N, Tavares L, Antunes F, Saldanha C. (2006) Changes in blood cell membrane properties in HIV type-1-infected patients. AIDS Res Hum Retroviruses. 22(9), 849-53.
      • Carvalho FA, Maria AV, Braz Nogueira JM, Guerra J, Martins-Silva J, Saldanha C. (2006) The relation between the erythrocyte nitric oxide and hemorheological parameters. Clin Hemorheol Microcirc. 35(1-2), 341-7.
      • Gonçalves S, Maria AV, Silva AS, Martins-Silva J, Saldanha C. (2006) Phytosterols in milk as a depressor of plasma cholesterol levels: experimental evidence with hypercholesterolemic Portuguese subjects. Clin Hemorheol Microcirc. 35(1-2), 251-5.
      • Silva AS, Saldanha C, Martins-Silva J. (2006) Effects of low-fat milk enriched with phytosterols on plasma cholesterol concentrations and hemorheological parameters of Wistar rats. Clin Hemorheol Microcirc. 35(1-2), 245-50.
      • Gonçalves S, Santos NC, Martins-Silva J, Saldanha C. (2006) Fibrinogen-beta-estradiol binding studied by fluorescence spectroscopy: denaturation and pH effects. J Fluoresc. 16(2), 207-13.
      • Carvalho FA, Graça LM, Martins-Silva J, Saldanha C. (2005) Biochemical characterization of human umbilical vein endothelial cell membrane bound acetylcholinesterase. FEBS J. 272(21), 5584-94.
      • Santos NC, Martins-Silva J, Saldanha C. (2005) Gramicidin D and dithiothreitol effects on erythrocyte exovesiculation. Cell Biochem Biophys. 43(3), 419-30.
      • Sargento L, Saldanha C, Monteiro J, Perdigão C, e Silva JM. (2005) Long-term prognostic value of protein C activity, erythrocyte aggregation and membrane fluidity in transmural myocardial infarction. Thromb Haemost. 94(2), 380-8.
      • Santos NC, Martins-Silva J, Saldanha C. (2005) PTEN "meets" DMSO. Leuk Res. 29(4), 361-2.
      • Lund, E., Guttinger, S., Calado, A., Dahlberg, J.E., Kutay, U. (2004) Nuclear export of microRNA precursors. Science 303: 95-98.
      • Rosário HS, Waldo SW, Becker SA, Schmid-Schönbein GW. (2004) Pancreatic trypsin increases matrix metalloproteinase-9 accumulation and activation during acute intestinal ischemia-reperfusion in the rat. Am J Pathol. 164: 1707-16.
      • Carvalho, F.A., Martins-Silva, J., Saldanha, C. (2004) Amperometric measurements of nitric oxide in erythrocytes. Biosensors and Bioelectronics 20 (3): 505-508. 
      • Carvalho FA, Mesquita R, Martins-Silva J, Saldanha C. (2004) Acetylcholine and choline effects on erythrocyte nitrite and nitrate levels. J Appl Toxicol. 24(6), 419-27.
      • Fitzal F, Delano FA, Young C, Rosario HS, Junger WG, Schmid-Schönbein GW. (2003) Pancreatic enzymes sustain systemic inflammation after an initial endotoxin challenge. Surgery 134: 446-56. 
      • Waldo SW, Rosario HS, Penn AH, Schmid-Schönbein GW. (2003) Pancreatic digestive enzymes are potent generators of mediators for leukocyte activation and mortality. Shock 20: 138-43. 
      • Santos, N.C., Figueira-Coelho, J., Martins-Silva, J., Saldanha, C. (2003) Multidisciplinary utilization of dimethyl sulfoxide: pharmacological, cellular, and molecular aspect. Biochem. Pharmacol. 65: 1035-1041.
      • Santos, N.C., Silva, A.C., Castanho, M.A.R.B., Martins-Silva, J., Saldanha, C. (2003) Evaluation of lipopolysaccharide aggregation by light scattering spectroscopy. ChemBioChem 4: 96-100.
      • Fitzal F, DeLano FA, Young C, Rosario HS, Schmid-Schönbein GW. (2003) Pancreatic protease inhibition during shock attenuates cell activation and peripheral inflammation. J. Vasc. Res. 39: 320-9.
      • Calado, A., Treichel, N., Muller, E.C., Otto, A., Kutay, U. (2002) Exportin-5-mediated nuclear export of eukaryotic elongation factor 1A and tRNA. EMBO J. 21: 6216-6224.
      • Santos, N.C., Figueira-Coelho, J., Saldanha, C. et al (2002) Biochemical, biophysical and haemorheological effects of dimethylsulphoxide on human erythrocyte calcium loading. Cell Calcium 31 (4): 183-188.

       

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